Which company is best for stem cell preservation?
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Both are good candidates for cell-based therapies using stem cells. | |
Thus, they are considered a promising stem cell source for therapeutic use. | |
This method may provide a new platform for stem cell culture for regenerative medicine. | |
167 Citations | We thus can suggest and advise for and against special combinations for stem cell-based tissue engineering. |
Related Questions
How FRIL helps in stem cell preservation?4 answersFlt3 receptor-interacting lectin (FRIL), extracted from Dolichos Lablab, plays a significant role in stem cell preservation by maintaining the quiescence and vitality of stem cells without inducing proliferation or differentiation. FRIL's effectiveness has been demonstrated in preserving hematopoietic stem cells (HSCs) and neural progenitor cells (NPCs) in vitro. In the case of HSCs, FRIL supplementation in serum-free suspension culture media has been shown to preserve these cells for extended periods, up to a month, by inhibiting both cell proliferation and differentiation, thereby maintaining their quiescence and potential for clinical applications. This preservation effect is further supported by the ability of FRIL to maintain cord blood-derived progenitors, indicating its potential for clinical hematology without the need for exogenous growth factors.
The mechanism through which FRIL preserves stem cell quiescence involves the modulation of cell cycle regulators. Specifically, FRIL down-regulates the expression of cyclins and CDKs associated with the G0/G1 phase, while up-regulating the tumor suppressor protein P53. This regulation helps in maintaining the stem cells in a quiescent state, preventing unnecessary proliferation or differentiation. Additionally, FRIL's impact on the expression of novel hematopoietic cell cycle regulators, HTm4 and HTm4S, further elucidates its role in stem cell preservation. The modulation of these regulators by FRIL contributes to maintaining a low proliferation rate of progenitor cells and preserving a higher number of primitive cells with proliferative potential in suspension culture.
In summary, FRIL aids in stem cell preservation by maintaining cell quiescence through the modulation of cell cycle regulators and the inhibition of proliferation and differentiation. This makes FRIL a valuable tool for extending the time and range of ex vivo manipulations of human stem cells for clinical applications, offering a cost-effective method for stem cell maintenance without the need for exogenous growth factors.
What are the different methods of cell preservation?3 answersCell preservation methods can be categorized into two main types: hypothermic preservation and cryopreservation. Hypothermic preservation involves storing cells at temperatures between 1°C and 35°C to maintain short-term cell viability for diagnosis and transport purposes. This method focuses on regulating cellular ATP levels, stabilizing cellular membranes, balancing antioxidant defense systems, and supplying mimetic extracellular matrix (ECM) to prolong cell longevity. On the other hand, cryopreservation involves storing cells at temperatures between -196°C and -80°C to maintain long-term cell viability for therapeutic cell product storage. This method aims to protect cells from osmotic or ice injury and alleviate oxidative stress to ensure cell survival. Emerging alternatives for cell preservation include encapsulating cells within hydrogels and using specialized devices for transportation, which can overcome the limitations of traditional cryopreservation methods. Additionally, there are specific cell preservation solutions available, such as a cell preservation liquid containing albumin, low molecular heparin sodium, trehalose, and normal saline, and a cell preservation solution containing methanol, proclin300, EDTA, NaCl, acetic acid, albumin, glucose, normal saline, and chromium chloride.
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