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Why sds-page is used in medicine?

Protein tertiary structure

Gel electrophoresis

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SDS-PAGE is utilized in medicine for various purposes, including diagnosing diseases like malaria by detecting antibodies against Plasmodium through serum protein analysis . Additionally, SDS-PAGE has been crucial in identifying proteins like titin, aiding in the diagnosis of pathological processes in muscles and evaluating treatment effectiveness . The technique's ability to denature proteins and resolve them based on molecular weight is essential for studying protein structures and characteristics, despite losing functional details like noncovalently bound metal ions . Moreover, SDS-PAGE plays a significant role in visualizing proteins in gels, preparing samples, and analyzing protein content changes in medical contexts . Overall, SDS-PAGE's versatility in protein analysis makes it a valuable tool in medical research and diagnostics.

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Open access•Journal Article•DOI Serum protein profile of malaria patients through sds page method Saira Shams, Shabir Ahmed, Sultan Ayaz, E. F. Abd_Allah, Sumaira Shams, Sadaf Niaz, Irum Gul, Naila Gul, Riaz Ullah - Show less +8 more 05 Feb 2016-African Journal of Traditional, Complementary and Alternative Medicines	SDS-PAGE is used in medicine, specifically for malaria, to accurately detect and separate specific proteins like antibodies against Plasmodium, aiding in diagnosis and potential vaccine development.
Open access•Book Chapter•DOI Peculiarities of SDS-PAGE of Titin/Connectin Ivan M. Vikhlyantsev, Zoya A. Podlubnaya - Show less +1 more 05 Nov 2018	SDS-PAGE is used in medicine to analyze titin content in muscles, diagnose pathological processes, and evaluate effective treatment approaches due to its ability to visualize high molecular weight isoforms.
Posted Content•DOI SDS PAGE Protocol -MChem Project v1 Kyu Cho 04 Mar 2022	SDS-PAGE is used in medicine to analyze proteins based on molecular weight, aiding in understanding protein structures and functions, crucial for medical research and diagnostics.
Book Chapter•DOI To identify a protein sample using SDS-PAGE 01 Jan 2023	SDS-PAGE is used in medicine to separate intricate protein mixtures, denature proteins, and determine covalent structural characteristics, aiding in protein identification and analysis in medical research.
Open access•Posted Content•DOI SDS-PAGE v2 A. V. Pirotskaya 11 Oct 2022	SDS-PAGE is used in medicine to visualize proteins, aiding in various medical research and diagnostic applications by separating and analyzing protein samples accurately.

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Can non denatured proteins loaded on sds page?5 answersNon-denatured proteins can be loaded on SDS-PAGE. However, the traditional SDS-PAGE method denatures proteins, resulting in the loss of their native molecular structures and biological activities. To address this limitation, a modified SDS-PAGE method called native (N)SDS-PAGE has been developed. This method retains the functional properties of proteins while still allowing for high-resolution separation. By changing the conditions of SDS-PAGE, such as removing SDS and EDTA from the sample buffer and reducing the concentration of SDS in the running buffer, native proteins can be separated and their functional properties can be retained. Additionally, blue-native (BN)-PAGE is another method that retains functional properties but at the cost of protein resolving power. Therefore, both native (N)SDS-PAGE and BN-PAGE can be used to separate non-denatured proteins and retain their functional properties.

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Is there a paper that supports clinicians with prescribing medicine?4 answersProviding decision support for the medical prescribing process remains a challenge and complicated task. The implementation of Clinical Decision Support System (CDSS) in clinical practice today is not fully integrated and achieved. The process of electronic approach to writing and sending medical prescription promises to improve patient safety, health outcomes, maintaining patients privacy, promoting clinician acceptance and prescription security when compared with the customary paper method. One form of safe supply enlists clinicians to prescribe opioids so that people have access to drugs of known composition and strength. The aim of this study was to identify perceived barriers to appropriate antibiotic prescribing across different healthcare settings.

Why SDS-PAGE is useful when it used together iwth western blotting?5 answersSDS-PAGE is useful when used together with western blotting because it allows for the separation and visualization of proteins based on their molecular weight. This technique involves the use of a gel matrix that separates proteins based on their size, allowing for the identification and characterization of specific proteins of interest. Western blotting, on the other hand, involves the transfer of proteins from the gel onto a membrane, which is then probed with specific antibodies to detect the presence of the target protein. By combining SDS-PAGE with western blotting, researchers can first separate the proteins of interest based on size and then detect and characterize these proteins using specific antibodies. This combination of techniques provides a powerful tool for protein analysis and identification.

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