Showing papers on "ATP transport published in 1987"

Rapid filtration technique for metabolite fluxes across cell organelles.

Abstract: Publisher Summary This chapter describes rapid filtration technique. The advantage is that it allows temporal resolution of noncovalent binding and of transport processes without implying the use of a specific carder inhibitor. It is, however, restricted to the cases where the enzyme or membrane fragments can be immobilized or adsorbed within a filter. The rapid filtration technique prevents any buffer washing after the filtration. The experiments––ADP/ATP transport in potato mitochondria, Pi-Pi exchange in intact mitochondria and calcium-induced calcium release in sarcoplasmic reticulum––described to illustrate some applications of the rapid filtration technique. The kinetics of phosphate-phosphate exchange mediated by the Pi carrier of the mitochondrial membrane is studied. Calcium release from the sarcoplasmic reticulum membrane of skeletal muscle triggers the contraction of the muscle fiber. The rapid filtration technique is clearly very well adapted to the study of ion or metabolite transport.