Showing papers on "Bradford protein assay published in 1975"
TL;DR: Nine protein assay procedures based on the phenol, biuret, and picryl sulfonate reactions were compared as to their suitability for the determination of protein in microsomal preparations, and three methods are particularly recommended.
79 citations
TL;DR: The chapter explores that 2,4,6-trinitrobenzene sulfonic acid (TNBS) of a suitable quality is obtained commercially as a pale yellow crystalline material, and suggests that a dilution of 12 M HCl would be equally satisfactory.
Abstract: Publisher Summary This chapter discusses the measurement of protein in lipid extracts In the course of studies of organic-solvent-soluble proteins, a variety of classic protein assay methods have been found to be inapplicable or involving excessive effort to reduce interference as a result of the presence of lipids in the mixture The simplest resource, extracting the lipids away from the protein by the use of organic solvents, fails because of the solubility of the protein in organic solvents The chapter explores that 2,4,6-trinitrobenzene sulfonic acid (TNBS) of a suitable quality is obtained commercially as a pale yellow crystalline material It may be recrystallized from 1 M HCl or from 2-butanone-petroleum ether mixtures As the crystalline material may have a variable degree of hydration, the stock solution of 003 M TNBS in butanol (tertiary : secondary, 9:1 v/v) is conveniently diluted from a stronger solution after an alkalimetric check on its concentration Organic solvents and potassium tetraborate are the analytical reagent grade or equivalent The 6 M HCl used is the easily prepared 61 M azeotrope; a dilution of 12 M HCl would be equally satisfactory
6 citations