Showing papers on "Gentiana published in 1997"
TL;DR: It was conduded that the four Gentiana species were amenable to propagation in vitro, except in G. lutea, in which adventitious roots were induced by applying naphthaleneacetic acid.
Abstract: The growth of axillary shoots was initiated on nodal stem segments, excised from aseptically grown seedlings of Gentiana acaulis L., G. cruciata L., G. lutea L. and G. purpurea L. In later subcultures, a basal callus tissue developed on the shoots, giving rise to de novo formed buds. Optimum benzyladenine and indoleacetic acid combinations for shoot development were established. They were slightly different in the four species. From 35-70% of shoots rooted spontaneously, except in G. lutea, in which adventitious roots were induced by applying naphthaleneacetic acid. It was conduded that the four Gentiana species were amenable to propagation in vitro.
54 citations
TL;DR: The DNA extracted from hairy roots and from the roots of transgenic plants hybridized with the appropriate genomic probes in Southern blotting is taken as evidence of the stable genetic transformation in the 4 Gentiana species.
Abstract: Shoots of micropropagated Gentiana acaulis, G cruciata, G lutea, and G purpurea were inoculated with suspensions of Agrobacterium rhizogenes cells, strains ATCC 15834 or A4M70GUS Adventitious roots appeared at the sites of inoculation in all 4 species Root tips were excised and cultured on growth regulator-free media for 2-6 years They exhibited very high branching and plagiotropism Spontaneous bud initiation occurred in roots of G cruciata Roots of G lutea, G acaulis and G purpurea were cultured on media with high kinetin concentration, which induced the formation of friable callus tissues Only in G purpurea were these calluses organogenic Regenerated shoots of G cruciata and G purpurea gave rise to plants, that displayed the typical phenotypes of A rhizogenes-transformed plants: short internodes and rolled leaves In the roots of G acaulis and G cruciata, transformed with A rhizogenes A4M70GUS, a positive reaction with X-gluc indicated the activity of β-glucuronidase The DNA extracted from hairy roots and from the roots of transgenic plants hybridized with the appropriate genomic probes in Southern blotting This is taken as evidence of the stable genetic transformation in the 4 Gentiana species
47 citations
26 Sep 1997
TL;DR: The volatile fraction was affected by the solvent used through the formation of esters of organic acids from the plant, and the amarogentin and gentiopicroside concentrations remained the same.
Abstract: Several solvents have been investigated for the preparation of bitter compounds of gentian roots (Gentiana lutea L.) for food applications. The highest concentrations of the bitter compounds, amarogentin and gentiopicroside, were obtained with ethanol : water 55 : 45 (v/v), propylene glycol: water 30 : 70 (v/v) and ethanol: propylene glycol: water 20 : 20 : 60 (v/v/v). Enzyme treatment prior to solvent extraction gave a greater extract yield (3.5%) but the amarogentin and gentiopicroside concentrations remained the same. The volatile fraction was affected by the solvent used through the formation of esters of organic acids from the plant.
25 citations
Journal Article•
1 citations