Showing papers on "Paper chromatography published in 2008"
TL;DR: The data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may be due to activation of macrophage, and the transcribing level of inducible NO synthase, TNF-α, IL-1β and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2.
76 citations
TL;DR: A reaction between pyruvic acid and tryptophan, which produced yellow products, was found in this paper, and the isolation and identification of the compounds produced by this interaction were carried out by paper chromatography, UV, IR and NMR spectroscopy, and organic synthetic procedures.
Abstract: A reaction between pyruvic acid and tryptophan, which produced yellow products, was found in this investigation. The isolation and identification of the compounds produced by this interaction were carried out by paper chromatography, UV, IR and NMR spectroscopy, and organic synthetic procedures. Four compounds were isolated: three were identitied as 1,2,3,4-tetrahydroharman-1,3-dicarboxylic acid, an isomer of 1,2,3,4-tetrahydroharman-1,3-dicarboxylic acid and harman. The compound which was not fully identified, was a yellow polymer. The decrease in concentration of pyruvic acid and tryptophan due to this interaction was analyzed by an automatic organic acid analyzer and an automatic amino acid analyzer. It was found that the ratio of pyruvic acid to tryptophan used in this reaction was approximately 1.6:1. This reaction was found to proceed via a 1:1 molecular reaction between tryptophan and pyruvic acid to form 1,2,3,4-tetrahydroharman-1,3-dicarboxylic acid and its yellow isomer followed by the formation of a yellow polymer produced by the interaction of the yellow isomer of 1,2,3,4-tetrahydroharman-1,3-dicarboxylic acid and pyruvic acid.
7 citations
TL;DR: In this paper, the authors investigated the discoloration caused by the interaction between α-ketoglutaric acid and tryptophan and found that five compounds were formed by this interaction, but only five compounds in the highest concentration were isolated: three were blue, one green and one brown-yellow.
Abstract: This study involved an investigation of the discoloration found to be caused by the interaction between α-ketoglutaric acid and tryptophan. Many products were formed by this interaction, but only the five compounds in the highest concentration were isolated: three were blue, one green and one a brown-yellow. Unfortunately, due to instability, these compounds were not identified However, the concentration of both cu-ketoglutaric acid and tryptophan, utilized during the reaction, were analyzed The results indicated that the ratio of α-ketoglutaric acid to tryptophan utilized was approximately 3:1. From these results along with various identification procedures (paper chromatography, UV-Vis, and IR spectroscopy, and organic synthesis) a pathway is postulated for the formation of the colored compounds in this interaction.
4 citations
Patent•
28 Jan 2008
TL;DR: In this article, an instrument and associated method are disclosed for preparing samples for column chromatography, which includes the steps of applying microwave energy to a sample composition containing at least one solvent to encourage a chemical reaction and generate desired products, thereafter mixing an absorbent media with the sample to absorb the solvent, the media being compatible with liquid chromatography that will separate the expected products, being chemically inert to the expected product, and being added in an amount sufficient to provide a substantially dry mixture of the media and the sample, but less than an amount that overly broadens the resolution of the sample
Abstract: An instrument and associated method are disclosed for preparing samples for column chromatography. The method includes the steps of applying microwave energy to a sample composition containing at least one solvent to encourage a chemical reaction and generate desired products, thereafter mixing an absorbent media with the sample to absorb the solvent, the media being compatible with liquid chromatography that will separate the expected products, being chemically inert to the expected products, and being added in an amount sufficient to provide a substantially dry mixture of the media and the sample, but less than an amount that overly broadens the resolution of the sample during liquid chromatography, thereafter applying microwave energy to the dry mixture of the media and the sample to thereby encourage the solvent to evaporate under the influence of the microwave energy, and thereafter adding the dry mixture of the media and the remaining sample to a liquid chromatography column and separating the components of the remaining sample for identification and purification purposes.
3 citations
TL;DR: In this paper, a tetradentate set of N2O2 salicylaldehyde-amine-Ndione Schiff base was prepared by condensation with salicaldehyde, ethylenediamine, 2,4-dione and reduction with NaBH4.
Abstract: A tetradentate set of N2O2 salicylaldehyde-amine-N-dione Schiff base was prepared by condensation with salicylaldehyde, ethylenediamine, 2,4-dione and reduction with NaBH4. The ligand system was characterized by 1H-NMR and FT-IR spectroscopy and HPLC. Radiolabeling studies of the 99mTc-complex were performed using stannous ions as the reducing agent. The purity of the complex was determined by ascending solvent system on paper chromatography and instant thin-layer chromatography (ITLC). The yield of the complex was >90%. Biodistribution of the 99mTc-complex of the precursor was studied in rabbits. A significant uptake and retention of injected activity was observed in the liver and cleared through the bladder. A faint activity was also observed in kidneys. These results indicate that the proposed system may be suitable for development of a liver/spleen imaging agent for future clinical applications.
1 citations
30 May 2008
30 May 2008