Showing papers on "Skin Carcinoma published in 1986"

Epidermodysplasia verruciformis. Skin carcinoma containing human papillomavirus type 5 DNA sequences and primary hepatocellular carcinoma associated with chronic hepatitis B virus infection in a patient.

Abstract: In a case of epidermodysplasia verruciformis with impaired cell-mediated immunity and multiple skin cancers human papillomavirus type 5 (HPV5) DNA sequences were demonstrated in a cutaneous squamous cell carcinoma. HPV5 and HPV8 were detected in the benign disseminated skin lesions together with three newly characterized HPVs: HPV17, HPV19 and HPV24. A chronic infection with hepatitis B virus resulting in macronodular cirrhosis associated with a primary hepatocellular carcinoma was also acquired by this patient. This case provides an example of the circumstantial evidence which suggests that certain types of HPV are potentially oncogenic and stresses the importance of immune surveillance in the protection against virus-associated tumors.

Human papillomavirus DNA detected in squamous-cell carcinoma tissue of a patient with epidermodysplasia verruciformis.

Abstract: Previously, we reported on histological investigations of malignant cutaneous changes in a case of epidermodysplasia verruciformis (EV) with skin cancer [6]. This patient was a 34-year-old Japanese m a n who had numerous pityriasis versicolor (PV)-like lesions and flat warts on the sun-exposed areas of his body, including the chest, back and extremities, persisting since childhood (Fig. 1 a). His elder sister had similar lesions on the skin of the same areas. Most of them were PV-like lesions. We extracted human papillomavirus (HPV) DNAs from purified virus particle fraction obtained separately from flat warts and PV-like lesions. After these HPV DNAs had been treated with some restrictive endonucleases, four kinds of HPV DNAs were isolated from this patient: three HPV DNAs from PVlike lesions on the chest and back and one HPV DNA from flat warts on the arms. Each HPV DNA was cloned with pBR322. This patient has repeatedly undergone surgical excisions since tumors first developed on the chest and face at the age of 21 years. Recently, this patient also noticed a hard, enlarging, subcutaneous nodule in the glabella area (Fig. 1 b). The nodule was partially excised and diagnosed as invasive squamous-cell carcinoma. Total DNA was extracted from the carcinoma tissue fragment using the procedure previously published, and purified by osmium chloride, ethidium bromide gradients and by agarose gel electrophoresis [2]. With Barn HI the molecule was cut at the three sites and this generated three fragments (Fig. 2), identical with one of the kinds of HPV DNA isolated from PV-like lesions on the chest and back. The result shows