Showing papers on "Super oxide dismutase published in 1999"

Protective effect of quercetin in cisplatin-induced cell injury in the rat kidney

Abstract: Objectives: To assess the protective effect of quercetin, a plant bioflavonoid, on cisplatin (CisDDP) induced nephrotoxicity in rats. Methods: Effect of the simultaneous treatment of quercetin (20 mg/kg, i.p., once a week x5) on cisplatin (3mg/kg, i.p., once a week x 5 ) induced renal epitheleal damage and lipid peroxidation was studied in the kidney of rats. Levels of markers indicative of nephrotoxicity such as urea, uric acid and creatinine were assessed in the serum. Changes in body weight were noted every week. The total protein content, levels of lipid peroxides and the activities of Na+ K + ATPase, Ca 2+ ATPase and Mg2+ ATPase were also assayed in the kidney. The glutathione content and the activities of antioxidant enzymes such as super oxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-s-transferase were estimated in the kidney. Results: Cisplatin administration caused a significant increase in the levels of all serum biochemical indices. The levels of lipid peroxides (in terms of TBA reactants) were increased significantly in serum and kidney. The activities of Na+ K+ ATPase, Ca2+ ATPase and Mg2+ ATPase were decreased significantly in the kidney. The glutathione content and activities of antioxidant enzymes were decreased considerably in the kidney of cisplatin treated rats when compared to normal control. Quercetin administration per se did not cause any change compared to the controls. However concomitant treatment of quercetin with cisplatin showed considerable decrease in levels of markers for nephrotoxicity and lipid peroxides and increased ATPase activities compared to CisDDP treated group. Glutathione content and antioxidant enzyme activities were significantly increased . Conclusion: Quercetin has significant cytoprotective effect in cisplatin-induced renal tubular damage in vivo in rats.

Use of anabolic agents, anti-catabolic agents, antioxidant agents, and analgesics for protection, treatment and repair of connective tissues in humans and animals

Abstract: The present invention relates to compositions for the protection, treatment and repair of connective tissues in humans and animals comprising any or all of anabolic, anti-catabolic, anti-oxidant and analgesic agents, including aminosugars, S-adenosylmethionine, arachadonic acid, GAGs, including pentosan, collagen type II, tetracyclines or tetracycline-like compounds, diacerin, super oxide dismutase, L-ergothionine, one or more avocado/soybean unsaponifiables, and an analgesic, e.g., acetaminophen, and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

Use of anabolic agents anti-catabolic agents and antioxidant agents for protection treatment and repair of connective tissues in humans and animals

Abstract: The present invention relates to compositions for the protection, treatment and repair of connective tissues in humans and animals comprising any or all of anabolic, anti-catabolic, and anti-oxidant agents, including aminosugars, S-adenosylmethionine, arachadonic acid, GAGs, pentosan, collagen type II, tetracyclines or tetracycline-like compounds, diacerin, super oxide dismutase, and L-ergothionine and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

Changes in the level of protein and activity of Cu/Zn-superoxide dismutase in zinc deficient rice plant, Oryza sativa L.

Abstract: In order to clarify the physiological role of Zn in rice plants through the identification and characterization of proteins affected by Zn deficiency, changes in the peptide profile in Zn-sufficient and Zn-deficient rice meristems were examined by two-dimensional pI-SDS PAGE. The level of some peptides fluctuated under Zn deficiency. One of the peptides for which the spot intensity decreased in the profile derived from Zn-deficient rice meristems was identified as Cu/Zn super oxide dismutase (Cu/Zn-SOD) by amino-terminal sequencing. Decrease in the Cu/Zn-SOD activity and of the Cu/Zn-SOD protein content was observed based on a nitro blue tetrazolium reduction assay and immunoblotting analysis, respectively. These results indicate that Zn deficiency in rice causes a reduction of the activity of Cu/Zn-SOD through the decrease of the protein content and/or inactivation of the enzyme in the meristem.

Agents and methods for protection, treatment and repair of connective tissue

Abstract: The present invention relates to compositions for the protection, treatment and repair of connective tissues in humans and animals comprising any or all of anabolic, anti-catabolic, anti-oxidant and analgesic agents, including aminosugars, S-adenosylmethionine, arachadonic acid, GAGs, including pentosan, collagen type II, tetracyclines or tetracycline-like compounds, diacerin, super oxide dismutase, L-ergothionine, one or more avocado/soybean unsaponifiables, hydroxyproline and an analgesic, e.g., acetaminophen, and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

Resistance Induced by Biotic and Abiotic Inducers to Rice Bacterial Blight (Xanthomonas oryzae pv.oryzae) and Its Relation to Active Oxygen Metabolism in Rice Seedlings

Abstract: Two rice cultivars,Yushuinuo and Zhefu 8o2, with high resistance and susceptibility to rice bacterial blight respectively were used as materials. Their second leaves were pre-treated at the two-1eaf stage with paraquat(PQ),a strain of Xanthomonas oryzae pv. oryzae(Xoo )75-1 with weak viru1ence and Tiron (4, 5-dihydroxy-l, 3-benzene-disufonic acid)which can specifically react with o2. The challenge inoculation with a strain 76-25 of high viruIence was used at the three-leaf stage. The results showed that pre-treatment with PQ and 75-1 strain induced systematic resistance to strain 76-25, and increased the rate of o2-generation and MDA (malondialdehyde) content in the induced leaves. The change of o2 -generating rate was greatest at 24 h to 72 h after induction with PQ. The changes of MDA content in the induced leaves were later than that of o2-generating rate and the greatest was at 72 h after induction with both PQ and 75-l strain. However the activities of super oxide dismutase (SoD) in the induced leaves decreased after induction with 75-l strain,but increased after induction with PQ. Tiron showed a negative effect on the induced resistance to Xoocaused by 75-l strain and PQ. lt was specially found that the incompatible interaction between 75-1 strain and two rice cultivars turned into a similar compatible interaction when 75-l strain added o2 scavenger Tiron as an inducer.