Analytical-scale supercritical fluid extraction

[/ The unique properties of supercritical fluids have prompted their use for a variety of applications in the field of analyticai chemistry. Perhaps the most widely cited use of these compressed fluids has been in the field of chromatography, either as mobile phase eluents or as extraction solvents. This study examines the various modes in which supercritical fluid extraction (SFE) can be employed by the chromatographer. Extraction, solubilization, and fractionation conditions are predicted by the application of well-known solution thermodynamic principles. Experimental results are reported for the removal of lipid phases from natural products and the coextraction of pe,sticide moieties. Finally, a method of predicting the required mobile phase pressures for solubilizing and fractionating oligomeric mixtures in supercritical fluid chromatography is compared with literature data.

Fundamentals and Applications of Supercritical Fluid Extraction in Chromatographic Science

History of supercritical fluid chromatography: instrumental development.

Sample preparation for chromatographic separations: an overview

An alternative extraction method for the removal of fats from meat samples is described employing supercritical carbon dioxide as the extracting medium. Experiments conducted at 35-70 MPa and 80 “C have shown that dense COz is an effective agent for selective removal of fat from a variety of meat matrices. Results obtained on meats varying in fat content from 2 to 35% by weight indicate that over 96% of the theoretical fat content can be removed. .Rapid extraction fluxes are realized when comminution and dehydration are performed on the samples prior to extraction. The described technique generates minimal toxicological risk to laboratory personnel and eliminates disposal problems of residual solvents.

Extraction of fat tissue from meat products with supercritical carbon dioxide

The application of fluid extraction in combination with fluid chromatography with packed column and flame ionization detection is described. Fluid chromatographic equipment is shown. Applications of this system to drug characterization are demonstrated.

A. Gross

Papers

On‐line extraction and separation by supercritical fluid chromatography with packed columns