Showing papers by "Aaron Kaplan published in 1992"

Phenotypic Complementation of High CO2-Requiring Mutants of the Cyanobacterium Synechococcus sp. Strain PCC 7942 by Inosine 5′-Monophosphate

Abstract: The high CO2-requiring mutants of Synechococcus PCC 7942, D4 and R14, were obtained by deletion or inactivation (respectively) of an open reading frame immediately downstream of rbc (the operon encoding the subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase). These mutants exhibit photosynthetic characteristics similar to those of high CO2-grown wild type, unlike other cyanobacterial high CO2-requiring mutants, where the apparent photosynthetic affinity for inorganic carbon is approximately 2 orders of magnitude lower than that of the wild type. Sequence analysis and metabolic complementation of the mutants by inosine 5′-monophosphate identified this open reading frame as the cyanobacterial equivalent of purK, the eubacterial gene encoding subunit II of phosphoribosyl aminoimidazole carboxylase in the purine biosynthetic pathway. Exposure of high CO2-grown Synechococcus to low CO2 conditions led to the induction of transcription of purK. It is suggested that the high CO2-requiring phenotype of these mutants resulted from the defect in purine biosynthesis after exposure to low CO2. We also raise the possibility that the level of cellular purines is involved in the process of adaptation of cyanobacteria to low concentrations of CO2.

A Cyanobacterial Gene Encoding Peptidyl-Prolyl cis-trans Isomerase.

Abstract: The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.