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Adam J. Fletcher
Researcher at University of Dundee
Publications - 21
Citations - 2040
Adam J. Fletcher is an academic researcher from University of Dundee. The author has contributed to research in topics: Ubiquitin ligase & Viral replication. The author has an hindex of 16, co-authored 21 publications receiving 1690 citations. Previous affiliations of Adam J. Fletcher include University of Oxford & University College London.
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Journal ArticleDOI
HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency.
Torsten Schaller,Karen E. Ocwieja,Jane Rasaiyaah,Amanda J. Price,Troy Brady,Shoshannah L. Roth,Stéphane Hué,Adam J. Fletcher,KyeongEun Lee,Vineet N. KewalRamani,Mahdad Noursadeghi,Richard G. Jenner,Leo C. James,Frederic D. Bushman,Greg J. Towers +14 more
TL;DR: It is reported that HIV-1 capsid binds directly to the cyclophilin domain of Nup358/RanBP2, and inhibition of CypA reduced dependence on Nup 358 and the nuclear basket protein Nup153, suggesting that CypA regulates the choice of the nuclear import machinery that is engaged by the virus.
Journal ArticleDOI
HIV-1 evades innate immune recognition through specific cofactor recruitment
Jane Rasaiyaah,Choon Ping Tan,Adam J. Fletcher,Amanda J. Price,Caroline Blondeau,Laura Hilditch,David A. Jacques,David L. Selwood,Leo C. James,Mahdad Noursadeghi,Greg J. Towers +10 more
TL;DR: It is concluded that HIV-1 has evolved to use CPSF6 and cyclophilins to cloak its replication, allowing evasion of innate immune sensors and induction of a cell-autonomous innate immune response in primary human macrophages.
Journal ArticleDOI
CPSF6 defines a conserved capsid interface that modulates HIV-1 replication.
Amanda J. Price,Adam J. Fletcher,Torsten Schaller,Thomas S. Elliott,KyeongEun Lee,Vineet N. KewalRamani,Jason W. Chin,Greg J. Towers,Leo C. James +8 more
TL;DR: A novel protein-protein interface in the N-terminal domain of HIV-1 CA is determined by X-ray crystallography, which mediates both viral restriction and host cofactor dependence, and is highly conserved across lentiviruses and is accessible in the context of a hexameric lattice.
Journal ArticleDOI
Host Cofactors and Pharmacologic Ligands Share an Essential Interface in HIV-1 Capsid That Is Lost upon Disassembly.
Amanda J. Price,David A. Jacques,William A. McEwan,Adam J. Fletcher,Sebastian Essig,Jason W. Chin,Upul D. Halambage,Christopher Aiken,Leo C. James +8 more
TL;DR: It is shown that a multi-subunit interface formed exclusively within CA hexamers mediates binding to linear epitopes within cellular cofactors NUP153 and CPSF6, and is competed for by the antiretroviral compounds PF74 and BI-2.
Journal ArticleDOI
Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21
TL;DR: The stepwise ubiquitination mechanism catalyzed by TRIM21, as well as the various cofactors required, that allows these two antiviral activities to occur synchronously at the proteasome are uncovered.