Adrian A. Franke

TL;DR: In this paper, a fast, sensitive, and precise method is presented for the efficient extraction and quantitation of coumestrol, daidzein, genistein, formononetin, and biochanin A from foods by diode array reversed-phase HPLC analysis using flavone as internal standard.

TL;DR: A simple and fast procedure to extract and simultaneously hydrolyze phytoestrogens and their conjugates from food items, and a fast and selective high-performance liquid chromatography (HPLC) method for precise determinations of the most common dietary phy toestrogens genistein, biochanin-A, daidzeln, formononetin, and coumestrol using flavone as internal standard are developed.

TL;DR: In this article, high-pressure liquid chromatography with electrochemical, diode-array and/or mass spectrometric detection revealed concentrations ranging in foods as eaten from 4 to 801 milligrams for ascorbic acid and 172 to 905 milligram for citrus flavanones.

TL;DR: The proposed method was applied to monitor isoflavone levels in soy foods and in human plasma, urine and breast milk after challenge with roasted soybeans and implications of the presented results on the potential activity of is oflavones to prevent cancer by exposing newborn infants to these agents are discussed.

TL;DR: The results indicate that the urinary excretion rate of total isoflavonoids measured from overnight urine samples may reflect reasonably well the usual intake of soy foods in a population with a high level of soy food consumption.