Showing papers by "Ahmad Sukari Halim published in 2014"
TL;DR: The findings suggest that the combined application of chitosan scaffold and TGFβ1 in conjunction with SHED might be beneficial for in vivo bone regeneration.
41 citations
TL;DR: In this article, the effect of plasma forming gases such as argon (Ar), oxygen (O 2 ), air and argon-oxygen (Ar+O 2 ) mixture on improvement of the surfaces properties of LDPE film using direct current (dc) excited glow discharge plasma.
39 citations
TL;DR: Chitosan in combination with keratinocytes and adipose-derived stem cells may act as a vehicle for delivering cells, which would increase the proliferation of keratinocyte numbers and help complete recovery from injuries.
Abstract: In the field of tissue engineering and reconstruction, the development of efficient biomaterial is in high demand to achieve uncomplicated wound healing. Chronic wounds and excessive scarring are the major complications of tissue repair and, as this inadequate healing continues to increase, novel therapies and treatments for dysfunctional skin repair and reconstruction are important. This paper reviews the various aspects of the complications related to wound healing and focuses on chitosan because of its unique function in accelerating wound healing. The proliferation of keratinocytes is essential for wound closure, and adipose-derived stem cells play a significant role in wound healing. Thus, chitosan in combination with keratinocytes and adipose-derived stem cells may act as a vehicle for delivering cells, which would increase the proliferation of keratinocytes and help complete recovery from injuries.
37 citations
TL;DR: It is concluded that early detection and revascularization of traumatic vascular injuries is important but delayedRevascularization also produced acceptable results.
Abstract: A seven years retrospective study was performed in 45 consecutive vascular injuries in the extremities to investigate the pattern of injuries, managements and outcomes. Motor-vehicle accidents were the leading cause of injuries (80%), followed by industrial injuries (11.1%) and iatrogenic injuries (4.4%). Popliteal and brachial artery injuries were commonly involved (20%). Fifteen (33.3%) patients had fractures, dislocation or fracture dislocation around the knee joint and 6 (13.3%) patients had soft tissue injuries without fracture. Traumatic arterial transection accounted for 34 (75.6%) cases, followed by laceration in 7 (15.6%) and 9 (6.7%) contusions. Associated nerve injuries were seen in 8 (17.8 %) patients using intra-operative findings as the gold standard, both conventional angiogram (CA) and computerized tomography angiogram (CTA) had 100% specificity and 100% sensitivity in determining the site of arterial injuries. The mean ischemic time was 25.31 hours (4 - 278 hours). Thirty-three (73.3 %) patients were treated more than 6 hours after injury and 6 patients underwent revascularization after 24 hours; all had good collateral circulation without distal pulses or evidence of ischemic neurological deficit. The mean ischemic time in 39 patients who underwent revascularization within 24 hours was 13.2 hours. Delayed amputation was performed in 5 patients (11.1%). Of the 6 patients who underwent delayed revascularization, one patient had early amputation, one -had delayed amputation following infection and multiple flap procedures while the rest of the patients’ limbs survived. Joint stiffness was noted in 10 patients (22.2%) involving the knee joint, elbow and shoulder in two patients each. Infection was also noted in 5 patients (11.1%) with two of them were due to infected implants. Other complications encountered included nonunion (2 patients, 4.4%), delayed union (1 patient, 2.2%), limb length discrepancy (1 patient, 2.2%), hematoma (1 patient, 2.2%) and leaking anastomosis in one patient (2.2%). Volkmann’s ischemic contracture occurred in 3 (6.7%) patients. There was no complication noted in 8 (17.8%) patients Three patients (6.7%) died of whom two were not due to vascular causes. We conclude that early detection and revascularization of traumatic vascular injuries is important but delayed revascularization also produced acceptable results.
24 citations
TL;DR: It is shown that the novel oligochitosan is able to induce the increased expression of gpiibiiia and P2Y12, thus accelerating platelet aggregation in vitro, and could be clinically useful to activate platelets to reach hemostasis.
Abstract: Platelet membrane receptor glycoprotein IIb/IIIa (gpiibiiia) is a receptor detected on platelets. Adenosine diphosphate (ADP) activates gpiibiiia and P2Y12, causing platelet aggregation and thrombus stabilization during blood loss. Chitosan biomaterials were found to promote surface induced hemostasis and were capable of activating blood coagulation cascades by enhancing platelet aggregation. Our current findings show that the activation of the gpiibiiia complex and the major ADP receptor P2Y12 is required for platelet aggregation to reach hemostasis following the adherence of various concentrations of chitosan biomaterials [7% N,O-carboxymethylchitosan (NO-CMC) with 0.45 mL collagen, 8% NO-CMC, oligochitosan (O-C), and oligochitosan 53 (O-C 53)]. We studied gpiibiiia and P2Y12 through flow cytometric analysis and western blotting techniques. The highest expression of gpiibiiia was observed with Lyostypt (74.3 ± 7.82%), followed by O-C (65.5 ± 7.17%). Lyostypt and O-C resulted in gpiibiiia expression increases of 29.2% and 13.9%, respectively, compared with blood alone. Western blot analysis revealed that only O-C 53 upregulated the expression of P2Y12 (1.12 ± 0.03-fold) compared with blood alone. Our findings suggest that the regulation of gpiibiiia and P2Y12 levels could be clinically useful to activate platelets to reach hemostasis. Further, we show that the novel oligochitosan is able to induce the increased expression of gpiibiiia and P2Y12, thus accelerating platelet aggregation in vitro.
14 citations
TL;DR: Most of the IMP vessels are small; however, they are still reliable and safe for use as recipient vessels in selected Asian patients and most are located in the subcutaneous plane, which further facilitates dissection and also reduces recipient-site morbidity.
Abstract: Background The use of internal mammary perforators (IMPs) as recipient vessels in autologous free flap breast reconstruction has many additional benefits compared with the internal mammary or thoracodorsal vessels. Our goals were to analyze the characteristics of these vessels and to evaluate the reliability of using them in an Asian population. Methods Thirty-five consecutive patients were prospectively studied between November 2000 and December 2010. Twelve patients underwent muscle-sparing-2 transverse rectus abdominis musculocutaneous flap, and 23 had deep inferior epigastric perforator flap reconstructions. Results Internal mammary perforator vessels were used in 29 patients (83%). Most (75%) of the vessels were located in the subcutaneous plane, and 85% were in the second and third intercostal spaces. There were significantly more usable IMP vessels in the immediate than in the delayed reconstructions (P = 0.049). All the flaps in the study were successful despite 1 requiring emergency re-exploration because of venous congestion. Conclusions Most of the IMP vessels are small; however, they are still reliable and safe for use as recipient vessels in selected Asian patients. Most are located in the subcutaneous plane, which further facilitates dissection and also reduces recipient-site morbidity.
13 citations
TL;DR: O-C films were found to be noncytotoxic to human fibroblast cultures and histological examination proved that films are biocompatible.
Abstract: BackgroundThe physical and biological characteristics of oligochitosan (O-C) film, including its barrier and mechanical properties, in vitro cytotoxicity and in vivo biocompatibility, were studied ...
10 citations
TL;DR: A rare case of successful reconstruction of a large tibial defect using a previously fractured fibula as a free osteoseptocutaneous flap is reported, which to the authors' knowledge has never been reported.
Abstract: Introduction Reconstruction of major bone defects using free fibular transfer provides a good biological option in unsound situations. Most authors recommend selection of the recipient blood vessels outside the zone of injury to achieve successful free fibular transfer. Occasionally, in polytraumatized patients, the surgeon has to use a previously fractured fibula as a graft, with increased risk of inclusion of the injury zone that may lead to failure. Methods We report a rare case of successful reconstruction of a large tibial defect using a previously fractured fibula as a free osteoseptocutaneous flap. The innovative point in our case is the inclusion of the fracture site within the utilized segment, which to our knowledge has never been reported. Conclusions As long as the microsurgical principles are adhered to, the effect of the zone of injury on the graft viability should not be overwhelming.
5 citations
TL;DR: In this article, the physicochemical characterization of four different types of chitosan biomaterials, which are classified into N, O -carboxymethylchitosans (NO-CMC) and Oligo-Chitosus (O-C), is performed.
Abstract: Chitosan is of great interest because it is biocompatible, biodegradable and abundant in nature. Accurate characterization of modified chitosan biopolymers is essential to optimize their usage. In our present work, we have tested the physicochemical characterization of 4 different types of chitosan biomaterials, which are classified into N , O -carboxymethylchitosan (NO-CMC) and Oligo-Chitosan (O-C). We have employed Fourier Transform Infrared Spectroscopy (FTIR) to analyze the functional groups and Scanning Electron Microscopy (SEM) to examine the scaffold membrane properties of each biomaterial. The FTIR analysis confirmed that a large number of alterations were made towards the NO-CMC group of chitosan. Meanwhile, most of the bands observed in the O-C group can generally be found in the standard model of chitosan. Shifting of the carbonyl group is only noticed in O-C group, which distinguishes both chitosan groups at 1644.20–1633.69 cm -1 peak.The NO-CMC and O-C groups, which have compressive porous structures, are able to support tissue and cell adherence via mechanical strength. Chitosan biopolymers, which vary from different grades and forms, are performing best when their unique properties are optimized. Hence, the study of these structurally modified chitosans and their characterization is very important to correlate their usage and properties in various fields.
4 citations
01 Jan 2014
3 citations
TL;DR: Comparing RNA and protein extractions from tissue biopsy of cleft lip palate in both fresh and stabilized condition found low RNA concentration from stabilized tissue was found, however, there was no crucial issue of protein extraction, degradation or concentration.
Abstract: Cleft lip palate is a human congenital disorder worldwide and the study of this genetic disease requires molecular genetic analysis. This analysis required the use of nucleic acid and protein, thus good quality and quantity of its extraction is important. We are comparing RNA and protein extractions from tissue biopsy of cleft lip palate in both fresh and stabilized condition. Tissue morphology was also captured using Scanning Electron Microscopy (SEM) for any morphology differences. Tissue homogenization may destroy nucleic acid stability but not its morphology. Low RNA concentration from stabilized tissue was found. However, there was no crucial issue of protein extraction, degradation or concentration. Tissue morphology was slight different between normal and CL/P tissue.
01 Jan 2014
TL;DR: HERS cells are a unique population of epithelial cells in the periodontal ligament that can regenerate cementum and chitosan scaffold is biologically renewable, biodegradable, biocompatible, non-antigenic, and non-toxic.
Abstract: Destruction of the periodontium as a result of furcal perforation or periodontitis leads to the loss of the tooth if not treated properly (1, 2). Regeneration of cementum is an essential event which allows the reattachment of the periodontal ligament to the alveolar bone and root (3). SHED represent a promising cell source for tissue engineering. HERS cells are a unique population of epithelial cells in the periodontal ligament that can regenerate cementum (4). It has been reported that HERS cells can differentiate into cementoblast-like cells through the epithelial-mesenchymal transition (EMT), and are capable of controlling the cementoblastic differentiation of periodontal ligament stem cells (PDLSC) (5). Transforming growth factor-β1 (TGFβ1) plays a key role in controlling cell proliferation and differentiation (6). Chitosan scaffold is biologically renewable, biodegradable, biocompatible, non-antigenic, and non-toxic (7, 8). 2.0 Materials and Methods