Akihiro Hara

TL;DR: Results suggested that the HYG gene was not functional due to the nonuniversal usage of the CTG codon, and this mutated hygromycin resistance gene may be useful for direct selection of C. tropicalis transformants.

TL;DR: A novel and convenient method to construct a ura3 strain (ura3/ura3) of the asporogenous and diploid yeast, Candida tropicalis, that produces dicarboxylic acid is established and the allelic POX4 gene was successfully disrupted.

TL;DR: A comparative study was carried out on the biosynthesis of peroxisomal enzymes in the n -alkane-assimilating yeast Candida tropicalis 1098 and its dicarboxylic acid-producing mutant M2030, where the mutant strain showed a drastic decrease in protein levels in acyl-CoA oxidase and 3-ketoacyl- coA thiolase, constituting the yeast peroxISomal β-oxidation system.

TL;DR: An upstream activation sequence (UAS) which can induce transcription in response to n-alkane ( UAS(ALK) was identified on the promoter region of the peroxisomal 3-ketoacyl coenzyme A (CoA) thiolase gene of C. tropicalis.

TL;DR: A transformation system using the autonomously replicating plasmid in the n-alkane-assimilating and asporogenic diploid yeast, Candida tropicalis, was developed and comparison of ARSs among yeasts revealed that the consensus sequence found in S. cerevisiae was also present in C. tropicalis.