Showing papers in "Journal of Bioscience and Bioengineering in 1999"

TL;DR: Comparative successes in fungal PUFA production demonstrate microbial potential to synthesize high-value oils and provide the main stimulus for their applications.

TL;DR: This review reconsiders the concept of the alpha-amylase family in the light of the recent wealth of information on the structures, the catalytic mechanisms, and the classification of amylases and points out the significance and problems of the sequence-based classification of glycosyl hydrolases.

TL;DR: A new enzymatic method of synthesizing methyl ester from plant oil and methanol in a solvent-free reaction system was developed and it is anticipated that such plant oil methyl esters can be used as a biodiesel fuel in the future.

TL;DR: The formation kinetics of calcium alginate gel capsules is studied and it is shown that an increase in the concentration ofAlginate gives rise to a reduction in membrane thickness, while an increase of calcium chloride leads to the formation of a thicker film.

TL;DR: The effect of furfural on aerobic and anaerobic batch cultures of Saccharomyces cerevisiae CBS 8066 growing on glucose was investigated and a previously unidentified compound was detected during the conversion offurfural.

TL;DR: An organic solvent-stable lipase (LST-03 lipase) secreted into the culture broth of the Organic solvent-tolerant Pseudomonas aeruginosa L ST-03 was purified by ion-exchange and hydrophobic interaction chromatography in the presence of 2-propanol.

TL;DR: In vitro and in vivo coupling of the cyanob bacterial photosynthetic system with a clostridial hydrogenase via cyanobacterial ferredoxin was demonstrated in the presence of light, and hydrogen productivity by the mutant was improved when irradiated with monochromatic light of some wavelengths.

TL;DR: Anionic glucose oxidase (GOD) was assembled alternately with polycations, namely, poly(ethylenimine) (PEI) and poly(dimethyldiallyl-ammonium chloride) (PDDA) in the preparation of molecular films, and its activity was about 80% of native GOD, indicating that the immobilization did not cause significant denaturation.

TL;DR: Control of pH and dissolved oxygen to optimal levels was effective for improving the production rate and yield of cellulose, to achieve a high cellulose productivity of 0.3 g cellulose/l x h.

TL;DR: The present review provides an overview of the knowledge available for class IIa bacteriocins and discusses common features and recent findings concerning these substances.

TL;DR: Low yeast extract concentration and long fermentation time enhance the production of cycled structures by all the production methods studied.

TL;DR: Numerical cluster analyses of quinone profiles are useful for monitoring microbial population shifts in an ecosystem which is not amenable to conventional culture methods and molecular techniques.

TL;DR: The possibility of employing methylated crude palm oil (CPOE) as an extraction solvent to reduce end-product inhibition and to enhance solvent productivity in acetone-butanol-ethanol (ABE) fermentation was evaluated using oleyl alcohol as the standard butanol extractant.

TL;DR: The review analyzes the results of recent studies on the biochemistry of high-molecular inorganic poly-phosphates (PolyPs) to find out whether they are polyfunctional compounds, which are of great significance to the approach to biotechnological, ecological and medical problems.

TL;DR: It is demonstrated that RNases H are classified into two major families, Type 1 and Type 2RNases H, of which only the Type 2 enzymes are present in all living organisms, including bacteria, archaea, and eucaryotes, suggesting that they represent an ancestral form of RNase H.

TL;DR: Results indicate that succinate, fumarate and malate are mainly synthesized through the TCA cycle (oxidative direction) even in the presence of glucose at a concentration as high as 15%.

TL;DR: The results suggest that the primer and probe systems can detect a considerable proportion of bacteria which can degrade aromatic compounds via catechol cleavage pathways newly isolated from a variety of environments.

TL;DR: This is the first report of an extremely halophilic bacterium that produces canthaxanthin, and among the isolated strains, strain TM exhibited the highest carotenoid-producing ability.

TL;DR: The author gives his personal view on the reasons why this cofactor was so lately discovered and how the steps in its identification were made and strong indications exist to assume that this is not the end of the story since other quinone cofactors seem awaiting their discovery.

TL;DR: Interestingly, EPS production was not strictly coupled to growth; the influence of different culture conditions on EPS production could be studied independent of growth, and the linking between growth and EPS synthesis was studied.

TL;DR: It was found that pH control definitely affects mycelial cell growth and exopolysaccharide (EPS) production of theMycelial cultivation of Ganoderma lucidum and it was proved that bistage pH control retained the desirable morphologies of the mycelia during cultivation and resulted in low viscosity and yield stress of the culture broth.

TL;DR: Hydcarbon production kinetics of this alga in the linear growth phase was found to be growth-associated irrespective of the experimental conditions of illumination and preculture.

TL;DR: A novel beta-agarase was purified from an agar-degrading alkalophilic bacterium, Alteromonas sp.

TL;DR: A thermophile, strain 93, which degrades poly(L-lactic acid) (PLA) film was isolated from 144 soil samples obtained from different locations by cultivation using an enrichment culture medium at 60 degrees C and gel permeation chromatograms showed a marked decrease in the main peak and the appearance of a new peak in the low molecular weight region.

TL;DR: Strain H-559, identified as Lactococcus lactis subsp.

TL;DR: The structures, functions and roles of the recently characterized catabolic transposons in bacteria are described and the mobile catabolic elements that share structural similarity with the pathogenicity and symbiosis islands are described.

TL;DR: A novel analytical method for analysis of microbial quinones in activated sludge sample was developed with improved reliability compared to the conventional method, demonstrating that the reliability of the analytical results of the improved method is significantly higher than that of theventional method.

TL;DR: The amount of accumulated GUS protein in the transformants was paralleled by the difference in the level of transcripts, and the pattern of gene expression was not the same as that of the endogenous genes in the chloroplast.

TL;DR: The results demonstrated that the filtration capacity of the SCMR was sufficient for a continuous and rapid replenishment of molasses solution from the dense cell culture and, therefore, the perfusion culture system is considered to provide a low-cost process for continuous production of lactic acid from cheap resources.

TL;DR: It is suggested that pineapple juice and the waste material can be useful low-cost substrates for ethanol production by Z. mobilis without supplementation with expensive organic nitrogen complexes such as yeast extract and without the regulation of the pH during cultivation, leading to the reduction in the production costs.