Showing papers by "Akio Sugihara published in 2004"

Critical temperature for production of MAG by esterification of different FA with glycerol using Penicillium camembertii lipase

Abstract: Production of MAG by a lipase-catalyzed reaction is known to be effective at low temperature. This phenomenon can be explained by assuming that synthesized MAG are excluded from the reaction system because MAG, which have low m.p., are solidified at low temperatures. Consequently, MAG are efficiently accumulated and do not serve as the precursor of DAG. If this hypothesis is correct, the critical temperature for MAG production, defined as the highest temperature at which DAG synthesis is repressed, should depend on the m.p. of the MAG. Esterification of FFA with glycerol using Candida rugosa, Rhizopus oryzae, and Penicillium camembertii lipases produced MAG efficiently at low temperatures. However, Candida lipase showed very low esterification activity at high temperatures (>20°C), and Rhizopus lipase produced not only MAG but also DAG even at low temperatures. Meanwhile, P. camembertii lipase catalyzed synthesis of MAG only from FFA and glycerol at low temperatures, although the enzyme catalyzed synthesis of DAG from MAG in addition to synthesis of MAG at high temperatures. We thus studied the effect of temperature on esterification of C10−C18 FFA with glycerol using Penicillium lipase as a catalyst and determined the critical temperatures for production of MAG. The critical temperature for production of each MAG showed a linear correlation with m.p. of the MAG, which supported the hypothesis. In addition, because the m.p. of MAG are estimated from that of the constituent FA, the optimal temperature for production of MAG can be predicted from the m.p. of the FFA used as a substrate.

Method for purification of conjugated linoleic acid isomer and application of the same

Abstract: PROBLEM TO BE SOLVED: To provide a method for purification of conjugated linoleic acid isomer, especially cis-9, trans-11 conjugated linoleic acid and trans-10, cis 12 conjugated linoleic acid isomers (educts or glyceride esters), capable of purifying in high purity and high yield and usable for food products. SOLUTION: The method for purification of conjugated linoleic acid isomer characteristically comprises a selective reaction (hydrolysis or esterification) to the linoleic acid isomers in a reaction system not containing organic solvents in the presence of lipase. Glyceride of ≥70% to the cis-9, trans-11 conjugated linoleic acid and trans-10, cis 12 conjugated linoleic acid isomers is contained in the ester obtained in the method. The foods comprise the fat and health component (such as margarine, cheese, source, etc.), wherein the health component comprises at least one of the above conjugated linoleic acid isomers (educts or glyceride esters). COPYRIGHT: (C)2004,JPO&NCIPI

Conjugate fatty acid menthol ester and its production method

Abstract: PROBLEM TO BE SOLVED: To provide an ester of a conjugate fatty acid and menthol and to provide a production technique of the ester. SOLUTION: The conjugate fatty acid menthol ester is a menthol ester of a conjugate fatty acid (for example conjugate linoleic acid). The conjugate fatty acid menthol ester comprises mostly the same quantity of 9cis, 11trans-conjugate linoleic acid and 10trans, 12cis-conjugate linoleic acid, or the menthol ester comprises the relative higher content of 9cis, 11trans-conjugate linoleic acid or 10trans, 12cis-conjugate linoleic acid. In the manufacturing method of the conjugate fatty acid menthol ester, a conjugate fatty acid, the alkyl ester or glycerol ester reacts with menthol under the presence of lipase for esterification or interesterification, and the formed conjugate fatty acid menthol ester is collected. COPYRIGHT: (C)2004,JPO