Alain Zweibaum

TL;DR: Twenty human colon carcinoma cell lines were studied for their ability to develop some of the characteristics of the normal intestinal epithelium, e.g., epithelial polarity, presence of the actin-binding protein villin, or the occurrence of an enterocytic differentiation either when cultured under standard conditions, as for Caco-2 cells, or when grown in a glucose-free medium.

TL;DR: It is proposed that cells which are able to differentiate and which are the origin of the small proportion of differentiated cell types found in postconfluent cultures of the original cell line possess an advantage which allows them to be adaptable to "metabolic stress" conditions.

TL;DR: Results show that glucose interferes with the expression of sucrase-isomaltase in Caco-2 cells at the mRNA level.

TL;DR: The results show that the capacity of this subpopulation of HT‐29 cells to grow and differentiate in the absence of sugar is a stable characteristic, and suggest that glucose metabolism interferes with the program of differentiation of HT-29 cells.

TL;DR: In both populations significant or even high levels of MUC mRNAs are already present in early cultures, i.e. at a stage when the mature mucins are not yet detectable, suggesting that mucin maturation is a later event.