The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.

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Initial sequencing and analysis of the human genome.

https://www.biosyn.com/Images/ArticleImages/pdf/A%20simple.pdf

A simple method for displaying the hydropathic character of a protein

We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.

/pdf/dna-primers-for-amplification-of-mitochondrial-cytochrome-c-46m2jyijnx.pdf

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.

The complete sequence of the 16,569-base pair human mitochondrial genome is presented. The genes for the 12S and 16S rRNAs, 22 tRNAs, cytochrome c oxidase subunits I, II and III, ATPase subunit 6, cytochrome b and eight other predicted protein coding genes have been located. The sequence shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

Sequence and organization of the human mitochondrial genome

Short communication Construction and characterisation of a genomic PAC library of the intestinal parasite Cryptosporidium par6um 1

L'invention concerne un procede pour analyser un acide nucleique, consistant a diviser l'acide nucleique en de nombreux fragments d'une sequence donnee, et a amplifier de maniere selective ces fragments individuels pour realiser une cartographie au moyen d'une technique modifiee de HAPPY mapping.

Alan T. Bankier

Papers

Short communication Construction and characterisation of a genomic PAC library of the intestinal parasite Cryptosporidium par6um 1

Cartographie happy mapping