A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm

A flow cytometer (20) capable of utilizing an imaging system including a sensor (88) to determine various properties of the flow cytometer (20). Importantly, the imaging allows for determination of a drop delay time (154). Other characteristics that can be determined include at least the width of the stream, pressure of the stream, effect of charged droplets on other droplets (44), trajectory of the stream, resonant frequency, wavelengths, change in position of droplet break-off point (150), and others. An automatic warning system (180) can be used to alert an operator of an anomaly during setup or during normal operation. Furthermore, a mechanical interrupter can be used to shield a sort result from contamination by an incorrectly functioning stream. The cytometer (20) also allows for the disablement of the sort, particularly the charging and deflection systems. In addition, a more accurate system for detecting the speed of the stream can be used. The imaging system including a sensor (88) can allow for the removal of background noise and the monitoring of a change in a stream characteristic.

Method and apparatus for flow cytometry

A light beam is directed towards a surface along a direction normal to the surface. The surface is caused to move so that the beam scans the surface along a spiral path. An ellipsoidal mirror is placed with its axis along the surface normal to collect light scattered by the surface and any anomalies at the surface at collection angles away from the surface normal. In some applications, a lens arrangement with its axis along the surface normal is also used to collect the light scattered by the surface and any anomalies. The light scattered by the mirror and lenses may be directed to the same or different detectors. Preferably light scattered by the surface within a first range of collection angles from the axis is detected by a first detector and light scattered by the surface within a second range of collection angles from the axis is detected by a second detector. The two ranges of collection angles are different, with one detector optimized to detect scattering from large particles and defects and the other detector optimized to detect light from small particles and defects.

Process and assembly for non-destructive surface inspections

A particle detector has a sample area of cross section no in excess of about 2 mm for containing environmental fluid, a light source on one side of the sample area for directing a collimated or nearly collimated beam of light through the sample air or water so that part of the light beam will be scattered by any particles present in the air or water while the remainder remains unscattered, and a beam diverting device on the opposite side of the sample area for diverting or blocking at least the unscattered portion of the beam of light and directing at least part of the scattered light onto a detector. The detector produces output pulses in which each pulse has a height proportional to particle size and a pulse height discriminator obtains the size distribution of airborne particles detected in the air or water sample at a given time from the detector output. The detector may also include a device for discriminating between biological agents and inorganic particles.

Pathogen and particle detector system and method

Disclosed is a lateral flow quantitative assay method which can measure one or more analyte species at the same time, with high sensitivity. Also, the present invention relates to a strip which can measure one or more analyte species at the same time, with high sensitivity and a package in which the strip is integrated with a laser-induced surface fluorescence detector. The present invention can quantify multiple analytes with a minimum detection limit of pg/ml. Therefore, the present invention provides an advantage capable of quantifying a plurality of analytes at the same time using a simple lateral flow assay strip.

Lateral Flow Quantitative Assay Method and Strip and Laser-Induced Fluorescence Detection Device Therefor

Disclosed is a radiation collector apparatus and method primarily for counting and analyzing a flow of dilute particulate material, such as blood cells, sperm cells and the like, through the use of light detection. The radiation collector apparatus comprises a reflector chamber having an ellipsoidal reflector surface with a pair of elipsoidal foci defining a first focus, f 11 , and second focus, f 12 , and a second reflector surface with a primary focus, f 21 , positioned at the same point as focus f 12 , and a secondary focus, f 22 . The second reflector surface has the configuration of one of the conic sections of revolution. In operation the radiation collector apparatus is provided with an intensifed beam of light and a stream of particulate material aligned to intersect the intensifed beam of light at focus f 11 . Detectable light signals, after two reflections, are received in a focused beam by a photosensitive detector.

Ellipsoid-conic radiation collector and method

In an apparatus in which particles are passed through an optical sensing zone to measure their radiant energy distribution such as, for example, light scattering characteristics for the purpose of identifying the particles, means and a method are provided for deviating the collected light in accordance with predetermined different paths to a plurality of different photodetecting devices. The deviation is effected independently of collection by optical radiant energy transmitting or reflecting means. The different photodetecting devices enable the measurement of energy directed along the particular path which is identified with that device. The paths are established by the deviating means rather than permitted to evolve by the scattering phenomena themselves whereby the photodetecting devices can be located in convenient arrangements and may be conventional in construction. Different forms of the invention combine optical reflection or refraction for collecting the scattered radiant energy with optical reflection or refraction for deviation to achieve different advantages and results.

Apparatus and method for measuring the distribution of radiant energy produced in particle investigating systems

Disclosed is a radiation collector apparatus and method primarily for counting and analyzing a flow of dilute particulate material, such as blood cells, sperm cells and the like, through the use of radiation detection. The radiation collector apparatus comprises a reflector chamber having an ellipsoidal reflector surface with a pair of ellipsoidal foci defining a primary focus and secondary focus. A window is formed in the ellipsoidal reflector surface and is centered on a symmetry axis defined by the primary and secondary foci. In operation the radiation collector apparatus is provided with an intensified beam of radiation and a stream of particulate material aligned to intersect the intensified beam of radiation at one of the ellipsoidal foci. Detectable radiation, either directly or after one or more reflections, proceeds through the window in an organized beam for detection.

Ellipsoid radiation collector apparatus and method

In an apparatus in which particles which may include biological cells are passed through an optical sensing zone to measure their spatial radiant energy distribution for the purpose of identifying and/or characterizing the particles or cells, a structure and method are provided for increasing the usefulness of a geometric configuration of photovoltaic detectors such as a known assembly of generally concentric rings of such detectors usually including some wedge-shaped detectors. These are provided in a generally planar form and the invention provides structure which captures radiant energy in a flow cell of a particular type before projecting the energy to the detector. In particular an ellipsoid, paraboloid, hyperboloid or similar reflector which is symmetrical about its optical axis is utilized first to capture the radiant energy on angles which could include most forward and/or backward angles and all azimuthal angles and then deviating the same to the detector assembly mentioned. By moving the arrangement or assembly of detectors along the optical axis of the reflector the optimum distance is established for obtaining the greatest amount of information sought. Although the assembly of photovoltaic detectors may thereafter be left in place, it is preferred to substitute for said assembly a second highly economical deviating or reflecting device capable of capturing the radiant energy which would otherwise have fallen on the sensitive plane of the assembly of photovoltaic detectors and deviating the same along a plurality of different spaced paths to independent photodetecting devices.

Radiant energy reradiating flow cell system and method

There is disclosed an illuminator and collector apparatus and method for fluorescence analysis wherein illumination of a stream of particles produces fluorescent light. A reflector, having a concave reflector surface with a first focus disposed in the stream of particles, has reflected therefrom organized illuminating radiation, such radiation converging upon the first focus to stimulate the particles into producing the fluorescent light. The fluorescent light emanates from the first focus, a portion of which is reflected from the concave reflector surface. This fluorescent light which is focused on the second focus contains no (or negligible) amount of illuminating radiation and is collected for subsequent detection and processing.

Albert Brunsting

Papers

Ellipsoid-conic radiation collector and method

Apparatus and method for measuring the distribution of radiant energy produced in particle investigating systems

Ellipsoid radiation collector apparatus and method

Radiant energy reradiating flow cell system and method

Dark field illuminator and collector apparatus and method