A
Alison Hinchliffe
Researcher at Norwich Research Park
Publications - 8
Citations - 801
Alison Hinchliffe is an academic researcher from Norwich Research Park. The author has contributed to research in topics: Genome editing & Transformation (genetics). The author has an hindex of 5, co-authored 7 publications receiving 468 citations. Previous affiliations of Alison Hinchliffe include John Innes Centre.
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Journal ArticleDOI
Speed breeding is a powerful tool to accelerate crop research and breeding
Amy Watson,Sreya Ghosh,Matthew J. Williams,William S. Cuddy,James Simmonds,María-Dolores Rey,M. Asyraf Md Hatta,M. Asyraf Md Hatta,Alison Hinchliffe,A. Steed,Daniel Reynolds,Nikolai M. Adamski,Andy Breakspear,Andrey V. Korolev,Tracey Rayner,Laura E. Dixon,Adnan Riaz,William Martin,Merrill Ryan,David Edwards,Jacqueline Batley,Harsh Raman,Jeremy Carter,Christian Rogers,Claire Domoney,Graham Moore,Wendy Harwood,Paul Nicholson,Mark J. Dieters,Ian H. DeLacy,Ji Zhou,Cristobal Uauy,Scott A. Boden,Robert F. Park,Brande B. H. Wulff,Lee T. Hickey +35 more
TL;DR: It is demonstrated that speed breeding in fully enclosed, controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies and transformation.
Journal ArticleDOI
An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat ( Triticum aestivum L.)
Sadiye Hayta,Mark A. Smedley,Selcen U. Demir,Robert Blundell,Alison Hinchliffe,Nicola Atkinson,Wendy Harwood +6 more
TL;DR: A high-throughput, highly efficient and repeatable transformation system for wheat is reported and this system has been used successfully to introduce genes of interest, for RNAi, over-expression and for CRISPR–Cas9 based genome editing.
Book ChapterDOI
Agrobacterium-Mediated Transformation of Barley Immature Embryos
Alison Hinchliffe,Wendy Harwood +1 more
TL;DR: An efficient and routine transformation protocol for the spring barley Golden Promise is described, based on Agrobacterium-mediated inoculation of immature embryos, which has been widely used for overexpression and RNAi applications and more recently for CRISPR/Cas9 mediated genome editing.
Posted ContentDOI
Speed breeding: a powerful tool to accelerate crop research and breeding
Amy Watson,Sreya Ghosh,Matthew J. Williams,William S. Cuddy,James Simmonds,María-Dolores Rey,M. Asyraf Md Hatta,Alison Hinchliffe,A. Steed,Daniel Reynolds,Nikolai M. Adamski,Andy Breakspear,Andrey V. Korolev,Tracey Rayner,Laura E. Dixon,Adnan Riaz,William Martin,Merrill Ryan,David Edwards,Jacqueline Batley,Harsh Raman,Christian Rogers,Claire Domoney,Graham Moore,Wendy Harwood,Paul Nicholson,Mark J. Dieters,Ian H. DeLacy,Ji Zhou,Cristobal Uauy,Scott A. Boden,Robert F. Park,Brande B. H. Wulff,Lee T. Hickey +33 more
TL;DR: It is demonstrated that speed breeding in fully-enclosed controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies, and transformation.
Journal ArticleDOI
In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons.
TL;DR: In this paper, the authors describe successful, heritable gene targeting in barley at the target site of Cas9 using an in-planta strategy but fail to achieve the same using a wheat dwarf virus replicon to increase the copy number of the repair template.