Showing papers by "Anand Swaroop published in 1992"
TL;DR: Using subtraction cloning, a human cDNA, AS321, which is expressed in retina and retinoblastoma cell lines but not in any other tissue or cell line tested, is isolated and its C-terminal region contains a basic motif and a leucine zipper domain similar to the DNA binding proteins of the jun and fos oncoprotein family.
Abstract: Using subtraction cloning, we have isolated a human cDNA, AS321, which is expressed in retina and retinoblastoma cell lines but not in any other tissue or cell line tested. AS321 mRNA is detected in all cells of neural retina, with a high level of expression in photoreceptors. The polypeptide sequence deduced from the cDNA reveals consensus phosphorylation sites for protein kinase A and proline-directed protein kinase. Its C-terminal region contains a basic motif and a leucine zipper domain similar to the DNA binding proteins of the jun and fos oncoprotein family, and it shows a strong similarity to the product of an avian retroviral oncogene, v-maf. The gene for AS321 is conserved during evolution and is expressed in vertebrate retina. We propose to name the gene NRL (neural retina leucine zipper.
324 citations
TL;DR: The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of expressed sequence tags (ESTs) should reduce the repetitive isolation of constitutively expressed sequences as discussed by the authors.
67 citations
TL;DR: The product of a neural retina-specific gene, NRL, belongs to the "leucine zipper" family of DNA-binding proteins and has a strong similarity to the v-maf oncogene product and is a candidate gene for retinal diseases.
15 citations
01 Jan 1992
TL;DR: The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of expressed sequence tags (ESTs) should reduce the repetitive isolation of constitutively expressed sequences.
Abstract: Expressed sequence tags (ESTs) provide useful molecular landmarks for physical mapping and identify the position of an expressed region in the genome. The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of ESTs should reduce the repetitive isolation of constitutively expressed sequences. We report here the sequence tags from the 3’-end region of 58 new directionally cloned cDNAs from a subtracted human retinal pigment epithelium (RPE) cell line library. Eight of the cDNAs have been assigned to human chromosomes using PCR-based EST assays. Chromosomal mapping of subtracted RPE cDNA clones may also help in identifying candidate genes for inherited eye diseases.
3 citations
01 Jan 1992
TL;DR: In this article, the authors present a set of strategies for the problem of finding the best solution to a given set of problems in the context of a large number of different types of problems.
Abstract: T h e p r o d u c t o f a n e u r a l r e t i n a s p e c i f i c g e n e , N R L , b e l o n g s to t h e " l e u c i n e z i p p e r " f a m i l y o f D N A b i n d i n g p r o t e i n s a n d h a s a s t r o n g s i m i l a r i t y to the v m a f o n c o g e n e p r o d u c t . T h e N R L g e n e m a p s to h u m a n c h r o m o s o m e 14 b y S o u t h e r n b lo t a n a l y s i s o f g e n o m i c D N A f r o m a h u m a n r o d e n t s o m a t i c ce l l h y b r i d p a n e l . In s i tu h y b r i d i z a t i o n to m e t a p h a s e c h r o m o s o m e s h a s f u r t h e r s u b l o c a l i z e d the g e n e to the r e g i o n 1 4 q 1 1 . 1 q 1 1 . 2 . D 1 4 S 4 6 E has n o w b e e n a s s i g n e d to the N R L g e n e . Bec a u s e o f i t s spec i f i c p a t t e r n o f e x p r e s s i o n , N R L is a cand i d a t e g e n e for r e t i n a l d i s e a s e s . © 1992 Academic Press, Inc.