Association between protein profile and postmortem interval in human bone remains.

The decomposition process of human remains can be used to estimate the post-mortem interval (PMI), but decomposition varies due to many factors. Temperature is believed to be the most important and can be connected to decomposition by using the accumulated degree days (ADD). The aim of this research was to develop a decomposition scoring method and to develop a formula to estimate the PMI by using the developed decomposition scoring method and ADD. A decomposition scoring method and a Book of Reference (visual resource) were made. Ninety-one cases were used to develop a method to estimate the PMI. The photographs were scored using the decomposition scoring method. The temperature data was provided by the Royal Netherlands Meteorological Institute. The PMI was estimated using the total decomposition score (TDS) and using the TDS and ADD. The latter required an additional step, namely to calculate the ADD from the finding date back until the predicted day of death. The developed decomposition scoring method had a high interrater reliability. The TDS significantly estimates the PMI (R
2 = 0.67 and 0.80 for indoor and outdoor bodies, respectively). When using the ADD, the R
2 decreased to 0.66 and 0.56. The developed decomposition scoring method is a practical method to measure decomposition for human remains found on land. The PMI can be estimated using this method, but caution is advised in cases with a long PMI. The ADD does not account for all the heat present in a decomposing remain and is therefore a possible bias.

/pdf/the-development-of-a-post-mortem-interval-estimation-for-1auh8uzad5.pdf

The development of a post-mortem interval estimation for human remains found on land in the Netherlands

Microbial life beyond the grave: 16S rRNA gene-based metagenomic analysis of bacteria diversity and their functional profiles in cemetery environments.

Knowledge on the postmortem interval (PMI) of wild boar (Sus scrofa) carcasses is crucial in the event of an outbreak of African swine fever in a wild boar population. Therefore, a thorough understanding of the decomposition process of this species in different microhabitats is necessary. We describe the decomposition process of carcasses exposed in cages. Trial 1 compared a wild boar and a domestic pig (Sus scrofa domesticus) under similar conditions; Trial 2 was performed with three wild boar piglets in the sunlight, shade, or in a wallow, and Trial 3 with two adult wild boar in the sun or shade. The wild boar decomposed more slowly than the domestic pig, which shows that standards derived from forensic studies on domestic pigs are not directly applicable to wild boar. The carcasses exposed to the sun decomposed faster than those in the shade did, and the decomposition of the carcass in the wallow took longest. To assess the state of decomposition, we adapted an existing total body scoring system originally developed for humans. Based on our studies, we propose a checklist tailored to wild boar carcasses found in the field that includes the most important information for a reliable PMI estimation.

Estimating the Postmortem Interval of Wild Boar Carcasses.

Estimation of the postmortem interval based on the human decomposition process

Several studies have been conducted on decomposition patterns and rates of surface remains; however, much less are known about this process for buried remains. Understanding the process of decomposition in buried remains is extremely important and aids in criminal investigations, especially when attempting to estimate the post mortem interval (PMI). The aim of this study was to compare the rates of decomposition between buried and surface remains. For this purpose, 25 pigs (Sus scrofa; 45-80 kg) were buried and excavated at different post mortem intervals (7, 14, 33, 92, and 183 days). The observed total body scores were then compared to those of surface remains decomposing at the same location. Stages of decomposition were scored according to separate categories for different anatomical regions based on standardised methods. Variation in the degree of decomposition was considerable especially with the buried 7-day interval pigs that displayed different degrees of discolouration in the lower abdomen and trunk. At 14 and 33 days, buried pigs displayed features commonly associated with the early stages of decomposition, but with less variation. A state of advanced decomposition was reached where little change was observed in the next ±90-183 days after interment. Although the patterns of decomposition for buried and surface remains were very similar, the rates differed considerably. Based on the observations made in this study, guidelines for the estimation of PMI are proposed. This pertains to buried remains found at a depth of approximately 0.75 m in the Central Highveld of South Africa.

/pdf/a-comparison-between-decomposition-rates-of-buried-and-25k3w4mhsm.pdf

A comparison between decomposition rates of buried and surface remains in a temperate region of South Africa.

Burial of remains is an important factor when one attempts to establish the post-mortem interval as it reduces, and in extreme cases, excludes oviposition by Diptera species. This in turn leads to modification of the decomposition process. The aim of this study was to record decomposition patterns of buried remains using a pig model. The pattern of decomposition was evaluated at different intervals and recorded according to existing guidelines. In order to contribute to our knowledge on decomposition in different settings, a quantifiable approach was followed. Results indicated that early stages of decomposition occurred rapidly for buried remains within 7-33 days. Between 14 and 33 days, buried pigs displayed common features associated with the early to middle stages of decomposition, such as discoloration and bloating. From 33 to 90 days advanced decomposition manifested on the remains, and pigs then reached a stage of advanced decomposition where little change was observed in the next ±90-183 days after interment. Throughout this study, total body scores remained higher for surface remains. Overall, buried pigs followed a similar pattern of decomposition to those of surface remains, although at a much slower rate when compared with similar post-mortem intervals in surface remains. In this study, the decomposition patterns and rates of buried remains were mostly influenced by limited insect activity and adipocere formation which reduces the rate of decay in a conducive environment (i.e. burial in soil).

/pdf/decomposition-patterns-of-buried-remains-at-different-3dizweabtz.pdf

Decomposition patterns of buried remains at different intervals in the Central Highveld region of South Africa

A controlled three-dimensional ground penetrating radar monitoring study over simulated clandestine
graves was conducted near Pretoria, South Africa, in which the detectability of graves as a
function of post-burial interval was assessed, as this is of particular interest to local forensic investigators.
It was demonstrated that the site-specific environmental parameter (a clay-rich loamy soil
with poor drainage) and heavy seasonal rainfall (as confirmed by ground-penetrating-radar-derived
soil moisture estimates) drastically compromised the long-term grave detectability, especially when
adopting a three-dimensional depth slice analysis approach. It is also seen that the disturbed burial
zone is the major contributor to the total grave anomaly rather than the buried body due to the combination
of environmental parameters and the absence of buried artefacts. This paper also advocates
the combined use of different data representations (two-dimensional and three-dimensional) to
increase the likelihood of detecting subtle grave anomalies.

/pdf/a-controlled-monitoring-study-of-simulated-clandestine-1hxu8hl507.pdf

Anatulie Marais-Werner

Papers

A comparison between decomposition rates of buried and surface remains in a temperate region of South Africa.

Decomposition patterns of buried remains at different intervals in the Central Highveld region of South Africa

A controlled monitoring study of simulated clandestine graves using 3D ground penetrating radar