Showing papers by "Anders Björklund published in 1970"
TL;DR: Two prominent neurone systems in the hypothalamo-pituitary complex are demonstrated: one containing dopamine, the other containing noradrenaline, and the results suggest the involvement of a third neurone system, probably containing an indole derivative.
225 citations
TL;DR: Dopamine was found in a special type of small, intensely fluorescent cells (“SIF-cells”) located among the adrenergic ganglion cell bodies, most of the dopamine present in the sympathetic ganglia is probably stored in these cells.
Abstract: In addition to noradrenaline (about 1.3–1.5 μg/g) dopamine (about 0.2 μ/g) is present in the sympathetic chains of the cat and pig. By means of a recently developed microspectro-fluorimetric method, the cellular localization of these two catecholamines has been studied. Dopamine was found in a special type of small, intensely fluorescent cells (“SIF-cells”) located among the adrenergic ganglion cell bodies. Most of the dopamine present in the sympathetic ganglia is probably stored in these cells.
204 citations
Journal Article•
TL;DR: Results strongly indicate that this intraneuronal fluorogenic substance is not identical with a catecholamine or 5-hydroxytryptamine, and showed general features characteristic for many indolykthylamines.
Abstract: Intraneuronal monoamines have been studied with the histochemical fluorescence method of Falck and Hillarp in the rat spinal cord. The formaldehyde-inducedintrancuronal fluorophores have been analyzed with microspectrofluorometry in combination with drugs which deplete monoamines from the central nervous system. Three types of intraneuronal fluorescence were found. 1) A green fluorescence, with spectra identical with those of the catecholamine fluorophores, which was depleted by reserpine and α-methyl- m -tyrosine but not by p -chlorophenylalanine, was found. This fluorescence was most likely derived from Primary catecholamines. 2) A yellow fluorescence, with spectra identical with those of the 5-hydroxytryptamine fluorophore, which was depleted by reserpine and p -chlorophenylalanine but not by α-methyl- m -tyrosine, was found. This fluorescence was probably derived from 5-hydroxytryptamine. 3) A yellow to brownish-yellow fluorescence, with spectra clearly deviating from those of the catecholamine and the 5-hydroxytryptamine fluorophores, which was depleted by reserpine but not by p -chlorophenylalanine or α-methyl- m -tyrosine, was found. Moreover, this fluorescenceshowed upon ultraviolet irradiation a photodecomposition rate, which was markedly slower than that of the 5-hydroxytryptamine fluorophore. These results strongly indicate that this intraneuronal fluorogenic substance is not identical with a catecholamine or 5-hydroxytryptamine. The spectral characteristics of this third type of fluorescence showed general features characteristic for many indolykthylamines.
49 citations
TL;DR: In this article, it was shown that hydrogen acid catalyzes the formation of fluorophores in the histochemical condensation reaction between gaseous formaldehyde and certain phenylethylamines and indolylethylamine.
Abstract: Hydrochloric acid catalyzes the formation of fluorophores in the histochemical condensation reaction between gaseous formaldehyde and certain phenylethylamines and indolylethylamines. Thus, when th...
39 citations
TL;DR: D dopamine was found to be localized in the two pairs of bright fluorescent cell bodies, which were situated in the dorsal and the ventrocaudal parts of the ganglion, respectively, as well as in strongly fluorescent varicosities distributed throughout the ganglia.
39 citations
TL;DR: It is possible, judging solely from structural relations, that both the cell body and the cell apex (the release pole) receive a dual innervation.
Abstract: The innervation pattern of the intermediate lobe of the skate (Raja radiata) was studied with histological and fluorescence histochemical methods. Neurosecretory fibres, stained with i.a. pseudo-iso-cyanine, were found running in bundles in the central parts of the cell cords. They terminated partly around the perinuclear parts of the intermedia cells, partly around the apices of the cells close to the vascular walls.
26 citations
TL;DR: It is suggested that silica gel thin layer can be used as a convenient model system for the histochemical reaction permitting a rapid and simple characterization of the resulting fluorophores and of the various fluorescent intermediate products in this reaction.
23 citations