Angeliki Öste Triantafyllou

TL;DR: The catalytic activities of lyophilized powders of alpha-chymotrypsin and Candida antarctica lipase were found to increase 4- to 8-fold with increasing amounts of either buffer salts or potassium chloride in the enzyme preparation and the enhancement of hydration level and flexibility of the macromolecule upon addition of the compounds partly provides the explanation for the observed results.

TL;DR: The effect of the addition of sorbitol on the activity and stability of enzymes was examined by monitoring transesterification reactions performed in organic media at various water activities, revealing that the chymotrypsin–sorbitol complex was stable against thermal denaturation, undergoing transition at a high temperature.

TL;DR: In this paper, a small amount of water (0.05%, v/v) was added to the reaction medium to increase the selectivity of the lipase and increased transesterification yields.