Showing papers by "Anne K. Vidaver published in 1986"
TL;DR: Production of syringacin W-1 is a 20 × 75 nm rod-shaped particle composed of an inner core and outer sheath that acts as a “spatially aggregating force” to form bacteriocin.
Abstract: Syringacin W-1, a bacteriocin produced by Pseudomonas syringae pathovar syringae strain PsW-1, is a 20 × 75 nm rod-shaped particle composed of an inner core and outer sheath. Production of syringac...
21 citations
TL;DR: Agarose gel elctrophoresis of restriction endonuclease digests of the phage DNAs indicated that the three phages are similar, but distinguishable in DNA structure.
Abstract: Bacteriophages Clmll, ClmX and ClmXC for Clavibacter (Cl.) michiganense subsp. nebraskense were characterized with respect to biological and physical properties. All phages produced clear plaques on strain CN18-5, the original propagating host. On strain CN76-2, ClmX produced predominantly turbid plaques with a few clear plaques, from which ClmXC was derived. The host range of the three phages was restricted to strains of Cl. michiganense subsp. nebraskense. Adsorption rate constants and burst sizes of the three phages were in the range of 2.9 to 9.4×10-9ml/min and 6 to 19 virions/cell, respectively. All phages were morphologically similar with a hexagonal head about 60nm in width and a flexuous tail approximately 235nm long. The type of nucleic acid was double-stranded DNA. Agarose gel elctrophoresis of restriction endonuclease digests of the phage DNAs indicated that the three phages are similar, but distinguishable in DNA structure. C1mXC was probably a deletion mutant of ClmX.
10 citations
TL;DR: The bacterium Clavibacter michiganense subsp.
Abstract: The bacterium Clavibacter michiganense subsp. nebraskense (Corynebacterium michiganense subsp. nebraskense) was grown in broth cultures and inoculated into corn plants. The plating efficiency of cells from broth cultures was essentially the same on nutrient broth-yeast extract and the semiselective medium for this bacterium, CNS. However, when cells were isolated from Goss bacterial wilt- and blight-infected corn, very few were recovered on CNS compared with the amount recovered on nutrient broth-yeast extract agar. When lithium chloride was omitted from the CNS, recoveries from infected corn were nearly the same as on nutrient broth-yeast extract agar. No other ingredient of CNS was inhibitory, nor did substitution of other salts for lithium chloride cause equal inhibition. The amount of inhibition was proportional to lithium chloride concentration. The inhibition by lithium chloride occurred with several strains of the bacterium isolated from one corn cultivar and with one of the strains recovered from three different cultivars of infected corn.
8 citations
TL;DR: Transposons Tn501 and Tn7 were introduced into extra-slow-growing Rhizobium japonicum by conjugal transfer of the 82 kilobase chimeric plasmid pUW942, and the formation of cointegrates between pUw942 and the chromosome of R.Japonicum was revealed.
Abstract: transconjugants were obtained atafrequency of10-7 to10-9. Thetransfer frequency ofstreptomycin resistance was lowerthanthatofmercuryresistance, andTn7 was relatively unstable. pUW942was notmaintained asan autonomously replicating plasmid inR.japonicum strains. However, some oftheHgrtransconjugants fromtheRJ19FY,RJ17W,andRJ12Sstrains acquired antibiotic markers ofthevector plasmid pUW942.Southern hybridization ofplasmid andchromosomal DNA ofR.japonicum strains with32P-labeled pUW942andpAS8Rep-l, thesame plasmid aspUW942except thatit doesnotcontain Tn501, revealed theformation ofcointegrates between pUW942andthechromosome ofR. japonicum. Moretransconjugants withonlyTn5OIinsertions inplasmids orthechromosome were obtained in crosseswithstrains RJ19FYandRJ17WthanwithRJ12S. Theseretained stable Hgrbothinplant nodules and undernonselective invitro growth conditions. OneoftheRJ19FYandtwooftheRJ12SHgrtransconjugants withvectorplasmid-chromosome cointegrates conjugally transferred plasmids of82,84or 86,and90 kilobases, respectively, into plasmidless Escherichia coli C.Theseplasmids strongly hybridized topUW942and EcoRIdigests oftotal DNA ofeachrespective R.japonicum strain butnottoindigenous plasmid DNA ofthe R.japonicumstrains. TheseR'plasmids consisted ofpUW942-specific EcoRIfragments andan additional one ortwonew fragments derived fromtheR.japonicum chromosome. Thegenetic analysis ofslow-growing rhizobia hasbeen hampered bythelackofsuitable genetransfer systems and apparent difficulties intheisolation ofauxotrophic mutants. Thegenetics oftheserhizobia arefurther complicated by spontaneous indigenous rearrangements between thechromosomeandplasmid DNA (2). We havebeeninterested in characterizing abiotype ofextra-slow-growing
5 citations