Anu Saloheimo

TL;DR: RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.

TL;DR: A novel yeast-based method to isolate transcriptional activators was applied to clone regulators binding to the cellulase promoter cbh1 of the filamentous fungus Trichoderma reesei, leading to the isolation of the cellulases activator ace2 encoding for a protein belonging to the class of zinc binuclear cluster proteins found exclusively in fungi.

TL;DR: The effect of deletion of the Trichoderma reesei ace1 gene encoding the novel cellulase regulator ACEI resulted in an increase in the expression of all the main cellulase genes and two xylanase genes in sophorose- and cellulose-induced cultures, indicating that ACEI acts as a repressor of cellulase and xylanases expression.

TL;DR: Xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae are modified by expression of heterologous and homologous permease-encoding genes and the new transporter homologue, Trxlt1 thus appears to code for a protein specific for xylOSE uptake.

TL;DR: A yeast expression library from the filamentous fungus Trichoderma reesel is constructed and a new β‐1,4‐endoglucanase gene is isolated on plates containing β‐glucan, a previously unknown small protein of 242 amino acids.