Copper metabolism of holstein and jersey cows and heifers fed diets high in cupric sulfate or copper proteinate.

The present invention provides a method for the determination of ceruloplasmin activity, wherein a member selected from the group consisting of (a) ferrocene or derivatives thereof (b) metallocene and (c) chelate metal containing iron or copper is reacted with a chromogen in the presence of ceruloplasmin to form colored material which is determined photometrically, as well as a reagent suitable therefor.

Method for the determination of ceruloplasmin activity

Disclosed is a method for detecting a diabetic peripheral vascular disorder such as diabetic macular edema, which comprises measuring at least one component selected from a polypeptide comprising an amino acid sequence depicted in any one of SEQ ID NO:1 to SEQ ID NO:9 shown in the Sequence Listing and a mutant or a fragment of the polypeptide in a biological sample collected from a subject.  Also disclosed is a composition or a kit for diagnosing and/or detecting a diabetic peripheral vascular disorder such as diabetic macular edema.

Method, composition and kit for detecting diabetic peripheral vascular disorder

PURPOSE:To obtain stable and highly active substrates for measuring the titled substance, by using 4-aminoantipyrine and an N-substitued aniline compound, or 3- methyl-2-benzothizolinone hydrazone and the N-substituted aniline compound as substrates. CONSTITUTION:4-Aminoantipyrine and an N-substituted aniline compound shown by the formula (R1 and R2 are lower alkyl group or 1-5C hydroxyalkyl group, etc; R3 and R4 are H, lower alkyl group, acetyl group, etc.), or 3-methyl-2-benzothiazolinone hydrazone and the N-substituted aniline compound shown by the formula as substrates are added to serum for measuring. After this, increase in absorption is determined, and the concentration of ceruloplasmin is calculated from a calibration curve.

Arai Yumi

Papers

Measurement of ceruloplasmin