Showing papers by "Arimichi Takabayashi published in 1995"

The ratio of reduced glutathione/oxidized glutathione is maintained in the liver during short-term hepatic hypoxia.

Abstract: Controversy persists as to whether reperfusion-induced injuries actually occur in the hepatocyte. The liver is the major source of glutathione, a scavenger of hydrogen peroxide. The aim of this study was to evaluate the sensitivity of the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) [GSH: GSSG] as an index of hepatic metabolic stress. A total of 121 rats were studied. The superior mesenteric vein (SMV) was occluded for 30 min, and this was followed by 0, 10, or 120 min of reperfusion. Total glutathione and GSSG levels in the liver, bile, and plasma were quantified, using glutathione reductase-coupled enzymatic assays. Results indicated that the hepatic GSH/GSSG ratio was maintained after an occulusion of the SMV, despite a decrease in adenosine triphosphate (ATP) level and energy charge potential. However, plasma levels of total glutathione and GSSG in the inferior vena cava increased after SMV occlusion and continued to increase after reperfusion. Biliary GSSG efflux decreased during 30-min occlusion of the SMV, and remained low even after reperfusion. The liver maintains homeostasis despite a decrease in biliary GSSG efflux, probably by secreting excess GSSG into the hepatic vein when the SMV is occluded. We conclude that the total amount of glutathione and GSSG in the plasma is directly correlated with oxidative stress in the liver.

Recurrent splenic artery aneurysms developing after aneurysmectomy without splenectomy: Report of a case

Abstract: We report herein the case of a 47-year-old man who developed a huge splenic artery aneurysm (SAA) with splenomegaly, for which ligation of the splenic artery and partial aneurysmectomy was performed. A celiac arteriogram taken 2 months postoperatively revealed that two small aneurysms had developed in the collateral vessels, indicating that increased blood flow through the collateral circulation could be responsible for the formation of secondary aneurysms. This postoperative change suggests that the etiology is related to the SAA and thus, the possibility that aneurysms may develop in the collateral vessels following spleen-preserving procedures for SAA must be borne in mind and careful follow-up performed at regular intervals.