Showing papers by "Asha J. Dissanayake published in 2023"
TL;DR: Xylochrysis aquatica as mentioned in this paper is a new taxon isolated from a decaying trunk of an undetermined wood, which differs from the type species X. lucida in having solitary, smaller ascomata with a short papillate ostiole, without a golden yellow layer of surrounded cells and larger asci with rounded ascospores.
Abstract: During surveys of ascomycetous fungi in Sichuan province, China, a new aquicolous taxon, Xylochrysis aquatica, isolated from a decaying trunk of an undetermined wood is described. Xylochrysis aquatica differs from the type species X. lucida in having solitary, smaller ascomata with a short papillate ostiole, without a golden yellow layer of surrounded cells and larger asci with rounded ascospores. The phylogenetic analysis of the combined ITS-LSU-SSU-rpb2-tef1-α sequence data matrix showed that X. aquatica clustered together with X. lucida and formed as different lineages, it can be recognized as a distinct species. A detailed description and an illustration of the novel species are given while a nucleotide comparison of related species is provided.
TL;DR: In this article , the major constraints in identifying plant pathogenic fungi (severely destructive on important crops) in sample collection, fi eld, and morphological observations, fungal isolation, obtaining pure cultures, applying Koch's postulates, DNA barcoding, phylogenetic analyses, and naming fungi (under "One fungus, One name" concept) are discussed.
Abstract: Fungal plant diseases are responsible for major crop and postharvest losses worldwide, with a signifi cant socio-economic impact on many individuals who depend on an agriculture-based economy. This review discusses the major constraints in identifying plant pathogenic fungi (severely destructive on important crops) in sample collection, fi eld, and morphological observations, fungal isolation, obtaining pure cultures, applying Koch’s postulates, DNA barcoding, phylogenetic analyses, and naming fungi (under “One fungus, One name” concept) based on selected case studies. Additionally, available strategies/methods to overcome those limitations up to date are also discussed. Ultimately, we proposed suggestions to minimize those limitations.
TL;DR: In this paper , the identification of 50 botryosphaeriaceous isolates was carried out based on both morphology and multi-gene phylogenetic analysis of internal transcribed spacer region (ITS), translation elongation factor 1-alpha gene (tef1) and β-tubulin gene (tub2).
Abstract: Abstract Woody oil plants are important economic trees which are widely cultivated and distributed throughout China. Surveys conducted during 2020 and 2021 on several woody oil plantations from five regions of Sichuan Province, China, revealed a high diversity of Botryosphaerialean fungi. The identification of 50 botryosphaeriaceous isolates was carried out based on both morphology and multi-gene phylogenetic analysis of internal transcribed spacer region (ITS), translation elongation factor 1-alpha gene (tef1) and β-tubulin gene (tub2). This allowed the identification of twelve previously known Botryosphaeriales species: Aplosporellaprunicola, A.ginkgonis, Barriopsistectonae, Botryosphaeriadothidea, Bo.fabicerciana, Diplodiamutila, Di.seriata, Dothiorellasarmentorum, Neofusicoccumparvum, Sardiniellaguizhouensis, Sphaeropsiscitrigena, and Sp.guizhouensis, and four novel species belonging to the genera Diplodia and Dothiorella, viz. Di.acerigena, Di.pistaciicola, Do.camelliae and Do.zanthoxyli. The dominant species isolated across the surveyed regions were Botryosphaeriadothidea, Sardiniellaguizhouensis and Diplodiamutila, representing 20%, 14% and 12% of the total isolates, respectively. In addition, most isolates were obtained from Pistaciachinensis (14 isolates), followed by Camelliaoleifera (10 isolates). The present study enhances the understanding of Botryosphaeriales species diversity on woody oil plants in Sichuan Province, China.