Showing papers by "Ashis Kumar Sen published in 2014"

Particle separation and sorting in microfluidic devices: a review

Abstract: Separation and sorting of micron-sized particles has great importance in diagnostics, chemical and biological analyses, food and chemical processing and environmental assessment. By employing the unique characteristics of microscale flow phenomena, various techniques have been established for fast and accurate separation and sorting of microparticles in a continuous manner. The advancements in microfluidics enable sorting technologies that combine the benefits of continuous operation with small-sized scale suitable for manipulation and probing of individual particles or cells. Microfluidic sorting platforms require smaller sample volume, which has several benefits in terms of reduced cost of reagents, analysis time and less invasiveness to patients for sample extraction. Additionally, smaller size of device together with lower fabrication cost allows massive parallelization, which makes high-throughput sorting possible. Both passive and active separation and sorting techniques have been reported in literature. Passive techniques utilize the interaction between particles, flow field and the channel structure and do not require external fields. On the other hand, active techniques make use of external fields in various forms but offer better performance. This paper provides an extensive review of various passive and active separation techniques including basic theories and experimental details. The working principles are explained in detail, and performances of the devices are discussed.

Hydrodynamic resistance and mobility of deformable objects in microfluidic channels.

Abstract: This work reports experimental and theoretical studies of hydrodynamic behaviour of deformable objects such as droplets and cells in a microchannel. Effects of mechanical properties including size and viscosity of these objects on their deformability, mobility, and induced hydrodynamic resistance are investigated. The experimental results revealed that the deformability of droplets, which is quantified in terms of deformability index (D.I.), depends on the droplet-to-channel size ratio ρ and droplet-to-medium viscosity ratio λ. Using a large set of experimental data, for the first time, we provide a mathematical formula that correlates induced hydrodynamic resistance of a single droplet ΔRd with the droplet size ρ and viscosity λ. A simple theoretical model is developed to obtain closed form expressions for droplet mobility ϕ and ΔRd. The predictions of the theoretical model successfully confront the experimental results in terms of the droplet mobility ϕ and induced hydrodynamic resistance ΔRd. Numerical simulations are carried out using volume-of-fluid model to predict droplet generation and deformation of droplets of different size ratio ρ and viscosity ratio λ, which compare well with that obtained from the experiments. In a novel effort, we performed experiments to measure the bulk induced hydrodynamic resistance ΔR of different biological cells (yeast, L6, and HEK 293). The results reveal that the bulk induced hydrodynamic resistance ΔR is related to the cell concentration and apparent viscosity of the cells.

Theoretical and numerical investigations of an electroosmotic flow micropump with interdigitated electrodes

Abstract: In this work, an electroosmotic flow micropump is proposed and investigated using theoretical analysis and numerical simulations. The micropump comprises an array of interdigitated electrodes on the top and the bottom surfaces of a rectangular microchannel. Theoretical analysis and extensive numerical simulations are performed to predict the pressure-flow characteristics of the micropump. The results of the model and simulations are compared which show good agreement with each other. The effects of various geometrical parameters including spacing between a pair of electrodes, gap between adjacent pairs of electrodes, width and height of the electrodes, and width of the microchannel and operating parameter including applied voltage on the performance of the micropump in terms of flow and pressure capacity is investigated.

Electrokinetic transport and separation of droplets in a microchannel

Abstract: This work presents theoretical, numerical and experimental investigations of electrokinetic transport and separation of droplets in a microchannel. A theoretical model is used to predict that, in case of micron-sized droplets transported by electro-osmotic flow, the drag force is dominant as compared to the dielectrophoretic force. Numerical simulations were performed to capture the transient electrokinetic motion of the droplets using a two-dimensional multi-physics model. The numerical model employs Navier–Stokes equations for the fluid flow and Laplace equation for the electric potential in an Arbitrary Lagrangian–Eulerian framework. A microfluidic chip was fabricated using micromilling followed by solvent-assisted bonding. Experiments were performed with oil-in-water droplets produced using a cross-junction structure and applying electric fields using two cylindrical electrodes located at both ends of a straight microchannel. Droplets of different sizes were produced by controlling the relative flow rates of the discrete and continuous phases and separated along the channel due to the competition between the hydrodynamic and electrical forces. The numerical predictions of the particle transport are in quantitative agreement with the experimental results. The work reported here can be useful for separation and probing of individual biological cells for lab-on-chip applications.