Astrid E. Visser

TL;DR: The findings demonstrate that early and late replicating foci are maintained during subsequent cell cycles as distinctly separated units of chromosome organization, compatible with the hypothesis that DNA organized into replicon clusters remains stably aggregated in non-S-phase cells.

TL;DR: It is shown that chromosome domains are either separated from one another by interchromatin space or are in close contact with no or little intermingling of their DNA, demonstrating that, while chromosomes form discrete territories, chromatin of adjacent chromosomes may be in contact in limited regions, thus implying chromosome-chromosome interactions.

TL;DR: In this paper, the boundary areas of individual chromosomes during interphase using a sensitive method based on replication labeling and immunocytochemistry were analyzed using confocal imaging of interphase nuclei revealed labeled chromosomal domains containing fiberlike structures and unlabeled areas.

TL;DR: It is established that the process of replication takes place in foci throughout the entire chromosome territory volume, in early as well as in late S-phase, demonstrating that activity of macromolecular enzyme complexes takes place throughout chromosome territories and is not confined to the territory surface as suggested previously.

TL;DR: Results show that the sites of DNA replication correspond essentially to perichromatin regions and that the newly replicated DNA moves rapidly from replication sites toward the interior of condensed chromatin areas.