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Astrid Kleen
Researcher at University of Hamburg
Publications - 2
Citations - 152
Astrid Kleen is an academic researcher from University of Hamburg. The author has contributed to research in topics: Glycoprotein & Glycopeptide. The author has an hindex of 2, co-authored 2 publications receiving 150 citations.
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Journal ArticleDOI
Mass Spectrometric Determination of the Sites of O-Glycan Attachment with Low Picomolar Sensitivity
Geert Jan Rademaker,Spiros A. Pergantis,Leonore Blok-Tip,James I. Langridge,Astrid Kleen,Jane Thomas-Oates +5 more
TL;DR: A sensitive protocol for unambiguously and positively identifying O-glycosylation sites in glycopeptides is described, based on beta-elimination of the glycan chain using NH4OH, and it is demonstrated that site determination can be achieved using as little as 1 pmol of starting material.
Journal ArticleDOI
Specificity of O-glycosylation by bovine colostrum UDP-GalNAc: polypeptide α-N-acetylgalactosaminyltransferase using synthetic glycopeptide substrates
Inka Brockhausen,Dale Toki,Jennifer Brockhausen,Stefan Peters,Stefan Peters,Tim Bielfeldt,Tim Bielfeldt,Astrid Kleen,Hans Paulsen,Morten Meldal,Fred K. Hagen,Lawrence A. Tabak +11 more
TL;DR: O-glycosylation by bovine colostrum ppGalNAc-T is a selective process dependent on both the amino acid sequence and prior glycosylations of peptide substrates, indicating charge-charge interactions of substrates with the enzyme.