Showing papers by "Ba-Bie Teng published in 2000"
TL;DR: Results indicate that apoB mRNA-specific hammerhead ribozyme can be used as a potential therapeutic agent to modulate apo B gene expression and to treat hyperlipidemia.
Abstract: In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To reduce the levels of apoB mRNA, we used adenovirus-mediated vector to target hammerhead ribozyme at GUA(6679) downward arrow of apoB mRNA (designated AvRB15) in the liver of a dyslipidemic mouse model that is deficient in apoB mRNA editing enzyme and overexpresses human apoB100. In this study, we delivered approximately 4 x 10(11) virus particles of AvRB15 (active ribozyme) or AvRB15-mutant (inactive ribozyme) to the animals. Using Southern blot analysis, we readily detected RB15 DNA in the mouse liver as long as day 35 after injection. This result was correlated with the RNA expression of RB15 by RNase protection assay. Using reverse ligation-mediated polymerase chain reaction, the 3' cleavage product of apoB mRNA was detected, and the exact cleavage site was confirmed by sequencing. Importantly, the levels of human and mouse apoB mRNA decreased approximately 80% after AvRB15 transduction. There was a marked decrease in plasma cholesterol, triglyceride, and human apoB of 42, 51, and 62%, respectively, when compared with the inactive ribozyme-treated group. Moreover, ribozyme cleavage of apoB mRNA generated a truncated protein of the expected size (apoB48.1), which was associated with lipoprotein particles in the very low density, low density, and high density lipoprotein fractions. Taken together, these results indicate that apoB mRNA-specific hammerhead ribozyme can be used as a potential therapeutic agent to modulate apoB gene expression and to treat hyperlipidemia.
20 citations
TL;DR: It is hypothesized that expression/activity of critical components of the apoptotic pathway can be used to induce apoptosis of a human prostate cell line derived from benign prostatic hyperplasia (BPH) tissue and it is postulated that overexpression and activation of a type II caspase should cause apoptosis.
20 citations
Patent•
17 Aug 2000
TL;DR: In this paper, a ribozyme for apolipoprotein B was proposed to treat a variety of conditions associated with atherosclerosis, including hypercholesterolemia and hyperlipidemia.
Abstract: The present invention is directed to ribozymes that specifically cleave mRNA for apolipoprotein B. The ribozyme may be used to treat a variety of conditions associated with atherosclerosis, including hypercholesterolemia and hyperlipidemia.
8 citations
Patent•
17 Aug 2000
TL;DR: In this article, a ribozyme for traiter une variete d'affections liees a l'atherosclerose, y compris l'hypercholesterolemie and l'Hyperlipidemie.
Abstract: L'invention concerne des ribozymes specifiques au clivage de l'ARNm de l'apolipoproteine B. Il est possible d'utiliser ce type de ribozyme pour traiter une variete d'affections liees a l'atherosclerose, y compris l'hypercholesterolemie et l'hyperlipidemie.