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Barry Falgout
Researcher at Center for Biologics Evaluation and Research
Publications - 23
Citations - 1406
Barry Falgout is an academic researcher from Center for Biologics Evaluation and Research. The author has contributed to research in topics: Dengue virus & Dengue fever. The author has an hindex of 15, co-authored 23 publications receiving 1367 citations. Previous affiliations of Barry Falgout include Office of Technology Transfer & Government of the United States of America.
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In vitro RNA synthesis from exogenous dengue viral RNA templates requires long range interactions between 5'- and 3'-terminal regions that influence RNA structure.
TL;DR: Results indicated that in vitro RNA synthesis at the 3′-untranslated region (UTR) required the presence of the 5′-terminal region (TR) and the two cyclization (CYC) motifs suggesting a functional interaction between the TRs.
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Identification of Specific Nucleotide Sequences within the Conserved 3′-SL in the Dengue Type 2 Virus Genome Required for Replication
TL;DR: This work examined the requirement for the 3′-SL in the context of dengue virus type 2 (DEN2) replication by mutagenesis of an infectious cDNA copy of a DEN2 genome and suggested that the wt DEN2 nucleotide sequence forming the bottom half of the long stem and loop in the 3″-SL was required for viability.
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A Live, Attenuated Dengue Virus Type 1 Vaccine Candidate with a 30-Nucleotide Deletion in the 3′ Untranslated Region Is Highly Attenuated and Immunogenic in Monkeys
Stephen S. Whitehead,Barry Falgout,Kathryn A. Hanley,Joseph E. Blaney,Lewis Markoff,Brian R. Murphy +5 more
TL;DR: RDEN1Δ30 was more attenuated in rhesus monkeys than the previously described vaccine candidate, rDEN1mutF, which also contains mutations in the 3′ UTR, and both vaccines were highly protective against challenge with wt DEN1.
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Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast
TL;DR: Intracellular dengue virus RNA from cells infected with transcript-derived virus contained an introduced BstEII site, proving that infectivity was derived from RNA transcripts and not from contamination with parental d Dengue virus.
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A Conserved Internal Hydrophobic Domain Mediates the Stable Membrane Integration of the Dengue Virus Capsid Protein
TL;DR: Results of in vivo expression and in vitro translation of wt and mutant forms of the DEN4 virion C demonstrated that the conserved internal hydrophobic segment in the DEN C functioned as a membrane anchor domain, and Signal peptide function was suggested by its requirement for the entry of C into membranes.