Bénédicte Gobert

TL;DR: It is shown that primary and secondary skeletal myogenesis can be recapitulated in vitro from the PSM-like cells, providing an efficient, serum-free protocol for the generation of striated, contractile fibers from mouse and human pluripotent cells.

TL;DR: An optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells (hPSCs) in vitro by mimicking key signaling events leading to muscle formation in the embryo is reported.

TL;DR: Pluripotent stem cells are used to generate early paraxial mesoderm and subsequently to recapitulate the stages of musculoskeletal progenitor specification and differentiation into muscle.

TL;DR: It is demonstrated that cells exhibiting characteristics of human fetal satellite cells can be produced in vitro from iPSC, opening interesting avenues for muscular dystrophy cell therapy and providing significant insights into the development of the human myogenic lineage.

TL;DR: A serum-containing protocol which parallels in vivo tissue maturation allowing differentiation of ES cells towards a paraxial mesoderm fate is described and provides an efficient system for the study of myogenic processes otherwise difficult to study in vivo such as fusion or satellite cell differentiation.