Solubilization of membranes by detergents

Solid lipid nanoparticles: Production, characterization and applications

The protein sequenator is an instrument for the automatic determination of amino acid sequences in proteins and peptides. It operates on the principle of the phenylisothiocyanate degradation scheme. The automated process embraces the formation of the phenylthiocarbamyl derivative of the protein and the splitting off of the N-terminal amino acid as thiazolinone. The degradation proceeds at a rate of 15.4 cycles in 24 hours and with a yield in the individual cycle in excess of 98%. The material requirements are approximately 0.25 μmoles of protein. The thiazolinones are converted to the corresponding phenylthiohydantoins in a separate operation, and the latter identified by thin layer chromatography. The process has been applied to the whole molecule of apomyoglobin from the humpback whale, and it has been possible to establish the sequence of the first 60 amino acids from the N-terminal end.

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1967.tb00047.x

A Protein Sequenator

Micromethod for the direct determination of serum triglycerides.

Dietary cholesterol consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, a risk factor for coronary heart disease. The molecular mechanism of sterol uptake from the lumen of the small intestine is poorly defined. We show that Niemann-Pick C1Like 1(NPC1L1) protein plays a critical role in the absorption of intestinal cholesterol. NPC1L1 expression is enriched in the small intestine and is in the brush border membrane of enterocytes. Although otherwise phenotypically normal, NPC1L1-deficient mice exhibit a substantial reduction in absorbed cholesterol, which is unaffected by dietary supplementation of bile acids. Ezetimibe, a drug that inhibits cholesterol absorption, had no effect in NPC1L1 knockout mice, suggesting that NPC1L1 resides in an ezetimibe-sensitive pathway responsible for intestinal cholesterol absorption.

https://science.sciencemag.org/content/sci/303/5661/1201.full.pdf

Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption.

/pdf/studies-of-intestinal-digestion-and-absorption-in-the-human-aktajizp4u.pdf

Studies of Intestinal Digestion and Absorption in the Human

Investigation on lipid separation methods. Separation of phospholipids from neutral fat and fatty acids.

/pdf/the-intraluminal-phase-of-fat-digestion-in-man-the-lipid-3k5hf02cnx.pdf

The intraluminal phase of fat digestion in man: the lipid content of the micellar and oil phases of intestinal content obtained during fat digestion and absorption.

Investigation on lipid separation methods. Separation of cholesterol esters, glycerides and free fatty acids.

http://www.farm.ucl.ac.be/FARM2129/2008-2009/Tulkens/SAR/Borgstrom-BBA-1988.pdf

Bengt Borgström

Papers

Studies of Intestinal Digestion and Absorption in the Human

Investigation on lipid separation methods. Separation of phospholipids from neutral fat and fatty acids.

The intraluminal phase of fat digestion in man: the lipid content of the micellar and oil phases of intestinal content obtained during fat digestion and absorption.

Investigation on lipid separation methods. Separation of cholesterol esters, glycerides and free fatty acids.

Mode of action of tetrahydrolipstatin: a derivative of the naturally occurring lipase inhibitor lipstatin.