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Bernard J. Mulligan
Researcher at University of Nottingham
Publications - 49
Citations - 1897
Bernard J. Mulligan is an academic researcher from University of Nottingham. The author has contributed to research in topics: Transformation (genetics) & Protoplast. The author has an hindex of 22, co-authored 49 publications receiving 1848 citations.
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The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes
Mark G. M. Aarts,Rachel Hodge,Kriton Kalantidis,Dion E. A. Florack,Zoe A. Wilson,Bernard J. Mulligan,Willem J. Stiekema,Rod J. Scott,Andy Pereira +8 more
TL;DR: The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product shows sequence similarity to a jojoba protein that converts wax fatty acids to fatty alcohols and a possible function of the MS2 protein as a fatty acyl reductase in the formation of pollen wall substances is discussed.
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Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts
Hongmei Zhang,H. Yang,Elíbio L. Rech,T. J. Golds,A. S. Davis,Bernard J. Mulligan,Edward C. Cocking,Michael R. Davey +7 more
TL;DR: This report of the production of kanamycin resistant transgenic rice plants establishes the use of protoplasts for rice genetic engineering.
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Microspore and pollen development in six male-sterile mutants of Arabidopsis thaliana
J. M. Dawson,Zoe A. Wilson,Mark G. M. Aarts,A. F. Braithwaite,L. G. Briarty,Bernard J. Mulligan +5 more
TL;DR: Five new recessive male-sterile mutants of Arabidopsis thaliana were isolated following seed mutagenesis by X-rays and ethyl methanesulfonate and implementation tests confirmed that the ms mutations were at different loci.
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Direct DNA transfer to plant cells
TL;DR: Direct DNA uptake is applicable to those plants not amenable to Agrobacterium transformation, particularly monocotyledons, and utilises a range of vectors, including those employed for gene cloning.
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Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.
Wen-Huei Chen,Kevan M.A. Gartland,Michael R. Davey,R. Sotak,J. S. Gartland,Bernard J. Mulligan,J. B. Power,Edward C. Cocking +7 more
TL;DR: Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 107 following PEG-induced uptake of Sma1 linearised pABD1 plasmid, and tissues maintained on this level of antibiotic expressed APH(3′)II activity, and contained DNA that hybridised to a probe with the APH (3′),II gene.