Willem J. Stiekema

TL;DR: A high-quality genome sequence of domesticated tomato is presented, a draft sequence of its closest wild relative, Solanum pimpinellifolium, is compared, and the two tomato genomes are compared to each other and to the potato genome.

TL;DR: The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes, suggesting that these genes form a lifestyle adaptation region in the chromosome.

TL;DR: Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases, and 54% of the predicted genes had significant similarity to known genes, and other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, and the frequent occurrence of clustered gene families.

TL;DR: Improvements over pBIN19 include location of the selectable marker gene at the left T- DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

TL;DR: The Hs1pro-1 locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet, which was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis.