Showing papers by "Bernhard Tribukait published in 1984"

Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study.

Abstract: The cellular DNA content in colorectal biopsies from 51 patients with chronic ulcerative colitis has been studied with flow-cytometric technique. The DNA pattern has been related to the duration of the disease, and to histopathology. Aneuploid cell lines were found in eight patients, two of which had histopathologically verified malignancies. Aneuploid cell lines were found in 2% of biopsies from mucosa classified as normal, in 10% of biopsies with inflammation, hyperplasia or atrophy, in 25% of polyps and in biopsies with dysplasia and in seven of eight biopsies from adenocarcinomas. Four of 27 cases with 11 to 20 years duration of disease, and 4 of 10 with more than 20 years disease, had aneuploid cell lines. Combined with colonoscopy the flow-cytometric technique can routinely be used in the follow-up of patients with ulcerative colitis in screening for malignant cell lines.

Chromosomal and DNA patterns in transitional cell bladder carcinoma. A comparative cytogenetic and flow-cytofluorometric DNA study.

Abstract: Biopsy tissue from 71 bladder tumors in 57 patients was studied by chromosome analysis and flow cytofluorometry. Chromosome analysis was hampered by preparation difficulties and was successful in only 53% of the analyzed tumors. DNA analysis failed to reveal near-diploid deviations, while the grossly aneuploid tumors generally had DNA values 10% to 15% above the numerical chromosome counts. Noninvasive tumors were diploid-near-diploid with occasional markers. Superficially invasive tumors were both diploid-near-diploid and near-tetraploid, but with a chromosome count of only 80 in the latter cases. Deeply invasive tumors tended to be triploid on DNA analysis, and had chromosome counts in the vicinity of 60. In addition to single chromosome abnormalities, increased malignancy apparently is also associated with an increased total number of chromosomes. A tetraploid DNA level seems to represent a more stable genome, although chromosomes show gross abnormalities including markers.

Flow DNA analysis of primary bone tumors. Relationship between cellular DNA content and histopathologic classification.

Abstract: substantial content of hard tissue. The results also indicate that DNA determinations as an adjunct to conventional histopathologic assessment may provide objective clinically relevant information with respect to the degree of malignancy. Thus, regardless of histogenetic origin, it appears that benign bone tumors

Clinical DNA flow cytometry.

Abstract: Modal DNA values of tumours from various sites may exhibit (1) a diploid-near diploid distribution, (2) an exponential distribution in the tetraploid to triploid range, or (3) a log-normal distribution in the triploid to tetraploid range. Examples of these various types of distribution are non-Hodgkin's lymphoma (1), aneuploid prostate carcinoma (2), aneuploid colon, breast, cervix and testicular carcinomas (3). These differences indicate dissimilarities in tumour development. In aneuploid tumours from the same site both tetraploid exponential and triploid-tetraploid log-normal distributions may occur. In bladder carcinoma these are related to grade. Modal DNA values in tumours are 10% higher than would be expected from modal chromosome numbers. This difference seems not to be due to a relative increase in large-sized chromosomes or due to technical shortcomings. Chromosome studies also show the possibility of the existence of near diploid malignant cells in grossly aneuploid tumours. Modal DNA values are connected with functional tumour properties by the proportion of S-phase cells. The significance of the latter is exemplified by follow-up of patients with bladder and cervix carcinoma.

DNA ploidy and cell phase in human pituitary tumors.

Abstract: The flow-cytofluorometric technique for nuclear DNA analysis was used in the study of 47 consecutive cases with pituitary tumors. The material consisted of 18 tumors secreting growth hormone (GH), 10 secreting GH and prolactin (PRL), 10 prolactinomas, 2 secreting thyroid-stimulating hormone (TSH), 2 secreting ACTH, and 5 were nonsecreting. An aneuploid DNA pattern occurred in 23 of 47 (49%) cases ranging from 1.4 to 3.6 N thus deflecting from the normal diploid 2N chromosome content of somatic nonreproductive cells. In four tumors two cell lines occurred, but one of them was always dominant, comprising 80% to 90% of all cells analyzed which totally amounted to 20,000 to 30,000 cells. The highest percentage of aneuploid tumors occurred in those with a concomitant secretion of GH and PRL (8 of 10, 80%). The percentage of diploid cells in tumors with an aneuploid nuclear DNA content was very low, 2.3 +/- 2.9% (+/- 0.65% SE). In 11 cases with aneuploidy, diploid cells were not detected. The mean age in patients with diploid and aneuploid tumors was rather similar in the different endocrinologic types of tumors.