Showing papers by "Bharat K. C. Patel published in 1987"
TL;DR: With the exception of serine, which could be catabolized as the sole carbon source, either peptone or yeast extract was essential for the fermentation of carbohydrates including glucose, maltose, mannose, xylan, starch, and pyruvate.
Abstract: Clostridium fervidus sp. nov. was isolated from a hot spring in New Zealand. The cells were strictly anaerobic, gram negative, sporeforming, and sluggishly motile rods (0.65 to 0.75 μ wide and 2 to 3 μm long). The spherical spores were subterminal to terminal and did not distend the sporangium. Lysis of the culture occurred at the onset of stationary phase. The deoxyribonucleic acid guanine-plus-cytosine content was 39 mol%. The temperature optimum was 68°C (range, > 37 and 5.5 and < 9.0). Growth occurred on Trypticase peptone (BBL Microbiology Systems) or yeast extract. However, with the exception of serine, which could be catabolized as the sole carbon source, either peptone or yeast extract was essential for the fermentation of carbohydrates including glucose, maltose, mannose, xylan, starch, and pyruvate. Acetate was always the major fermentation end product. CO2, H2, and minor quantitites of valerate, butyrate, ethanol, and lactate were also produced. C. fervidus (type strain Rt4-B1T) has been deposited with the American Type Culture Collection (ATCC 43204).
83 citations
TL;DR: A strain similar to Dictyoglomus thermophilum, isolated from a New Zealand hot spring, is described and growth was inhibited by penicillin, tetracycline and chloramphenicol indicating that the organism was a eubacterium.
Abstract: A strain similar to Dictyoglomus thermophilum, isolated from a New Zealand hot spring, is described. This strictly anaerobic, Gram-negative, non-motile and nonsporulating bacterium usually exists as long thin filaments of 5 to 25 μm by 0.35 to 0.45 μm. Rotund bodies are commonly observed. Thin sections of the cells revealed a two-layered cell wall. The optimum temperature and pH for growth was 70°C and 7.0 and 7.5 respectively. No growth was observed at 40°C and 85°C or at pH 4.5 to pH 9.0. The organism fermented glucose, maltose, mannose, xylose, lactose, cellobiose, galactose and sucrose and produced acetate as the major end-product with significant amounts of lactate, H2 and CO2 and only traces of ethanol. The doubling time on glucose was 10 h. The DNA base composition was 29.5% guanine plus cytosine as determined by the thermal denaturation method. Growth was inhibited by penicillin, tetracycline and chloramphenicol indicating that the organism was a eubacterium. These features are in common with the newly described species Dictyoglomus thermophilum to which the New Zealand isolate belongs.
27 citations
TL;DR: In this article, the characteristics of growth on starch were determined for 8 new isolates of thermophilic carbohydrate fermenting anaerobic bacteria and compared to some of the type cultures.
16 citations