Biliana Lesic

TL;DR: It is shown that HHQ is highly produced in vivo, where it is not fully converted into PQS, and it is demonstrated that it is required for MvfR‐dependent gene expression and pathogenicity; PqS is fully dispensable, as pqsH– mutant cells, which produce HHQ but completely lack P QS, display normal Mv fR‐ dependent genes expression and virulence.

TL;DR: These compounds are the first compounds identified to reduce the formation of antibiotic-tolerant persister cells and provide for the development of next-generation clinical therapeutics to more effectively treat refractory and deleterious bacterial-human infections.

TL;DR: In this article, the authors identified halogenated AA analogs that specifically inhibited HAQ biosynthesis and disrupted MvfR-dependent gene expression, without perturbing bacterial viability, and inhibited osmoprotection, a widespread bacterial function.

TL;DR: The contribution of the QS systems to T6S gene regulation is illustrated and the importance of HSI-II and -III in mediating P. aeruginosa pathogenesis is revealed, providing new insights into the design and development of selective compounds that may restrict the development of clinical infections.

TL;DR: The lambda Red-based technique can be used efficiently to generate mutants in P. aeruginosa by electroporating a polymerase chain reaction (PCR) fragment that carries an antibiotic cassette flanked by a region homologous to the target locus into a strain that expresses the lambda Red recombination system.