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Showing papers by "Biswanath Bhunia published in 2020"


Journal ArticleDOI
15 Apr 2020-Fuel
TL;DR: An attempt was taken for a stable generation of power from dairy wastewater using single chamber microbial fuel cell (SCMFC), and Escherichia coli-K-12 was used as a biological catalyst as mentioned in this paper.

34 citations



Journal ArticleDOI
TL;DR: A statistical optimization was performed to achieve a higher yield of cell growth and 4-chlorophenol (4-CP) biodegradation, where isolated Bacillus subtilis MF447840.1 was used as biocatalyst.
Abstract: A statistical optimization was performed to achieve a higher yield of cell growth and 4-chlorophenol (4-CP) biodegradation, where isolated Bacillus subtilis MF447840.1 was used as biocatalyst. Taguchi methodology was used to evaluate the optimum condition for eleven process parameters. Six media components such as ammonium nitrate, magnesium sulfate, dipotassium phosphate, monopotassium phosphate, calcium chloride, and trace element, and five physicochemical factors such as pH, temperature, agitation, inoculum size, and incubation time, were considered for optimization owing to their significant effect on cell growth and 4-CP biodegradation. Orthogonal Arrays (OA) layout of L27 was used for designing of various experimental trials. Signal to noise (S/N) ratio was used to evaluate the individual effects of process parameters and their optimal level during evaluation of cell growth and 4-CP biodegradation. Optimization studies revealed that 911.45 mg/L of biomass yield and 1000 mg/L of 4-CP biodegradation were found with ammonium nitrate (0.75 g/L), magnesium sulfate (0.25 g/L), dipotassium phosphate (0.75 g/L), monopotassium phosphate (0.25 g/L), calcium chloride (0.02 g/L), trace element (1%), pH (7.5), temperature (37 °C), agitation (150 rpm), inoculum size (10%), and incubation time (40 h).

17 citations


Journal ArticleDOI
TL;DR: Owing to its high potential in various sectors, there is an urgent need to explore and exploit the biosynthesis of prodigiosin using various biotechnological approaches and multifarious aspects in commercial-scale production of progetiosin along with its contemporary utilization have been critically assessed.
Abstract: Increasing utilization of artificial chemical dyes as coloring agent and their associated detrimental effects on human health have instigated the search for novel natural pigments for applications in the food and pharmaceutical industries. A wide range of advancements have been recorded for utilization of naturally extracted pigments, owing to their assurance in affirmative health attentiveness in the past few decades. Therefore, it is essential to investigate various potential natural resources such as plants and microbes from where food-grade colorants can be obtained. Several drawbacks such as low water solubility, instability to light, heat, or adverse pH are associated with natural pigments extracted from plants. On the contrary, microbial pigments having higher stability and productivity are considered a potential alternative to synthetic dyes. Prodigiosin is a linear tripyrrole natural red pigment obtained from Serratia marcescens which is produced as a secondary metabolite. Prodigiosin and their derivatives act as proapoptotic agents against diverse cancer cell lines and also play a vital role in numerous cellular targets, including multi-drug resistance cells with reduced or no reported adverse side effects. Owing to its high potential in various sectors, there is an urgent need to explore and exploit the biosynthesis of prodigiosin using various biotechnological approaches. Furthermore, multifarious aspects in commercial-scale production of prodigiosin along with its contemporary utilization have been critically assessed in this article.

17 citations


Journal ArticleDOI
TL;DR: In this paper, an attempt was taken in the present study for sustainable power generation from single chamber microbial fuel cell (SCMFC) using Pseudomonas aeruginosa-MTCC-7814.

15 citations


Journal ArticleDOI
TL;DR: Both exo-polysaccharide were found negative in charge and showed remarkable antioxidant and scavenging activities and exhibited the behaviour of pseudo-plastic fluids.

12 citations


Journal ArticleDOI
01 Jun 2020-Fuel
TL;DR: In this paper, the isolation of ten different cyanobacteria were carried out from northern parts of India and among them the cyanobacterium with maximum specific growth rate (0.246 days) was selected for the present study.

12 citations


Journal ArticleDOI
TL;DR: In this paper, an attempt was taken for rapid evaluation of iron-reduction capacity and growth of Shewanella algae through optimization of process parameters using Taguchi methodology, where five process parameters, including lactose, trace element, inoculum percentage, pH, and temperature, were taken into consideration as imperative factors and were optimized for evaluation of IR analysis along with growth of bacteria.
Abstract: Due to energy expansion and sustainable development of resources and systems, there has been an increasing demand for environmentally friendly alternative energy systems. Microbial fuel cells (MFCs) are considered one of the most promising alternative energy generation systems. The microorganism is one of the key limiting factors to enhance the performance efficiency of MFC. Therefore, proper evaluation of screening protocol is imperative benchmarks for selection of biocatalyst in MFC. In the present study, an attempt was taken for rapid evaluation of iron-reduction capacity and growth of Shewanella algae through optimization of process parameters using Taguchi methodology. Here, five process parameters, including lactose, trace element, inoculum percentage, pH, and temperature, were taken into consideration as imperative factors and were optimized for evaluation of iron-reduction analysis along with growth of bacteria. The main effect of each parameter, along with their interaction influence and optimal value, was determined using a signal-to-noise (S/N) ratio. The evaluation of quality characteristics of each parameter was also determined in Taguchi robust methodology using S/N ratio. Analysis of variance was performed to assess statistically important process parameters. Predicted results exhibited that enhanced bacterial growth (120%) and iron-reduction capacity (114%) can be achieved with 8 g/L of lactose, 2 mL of trace element solution, 7 of initial pH, 30 °C of temperature, and 4% (v/v) of inoculum percentage. It is evident from the results that Shewanella algae can be used as a promising catalyst for MFC.

10 citations