Separation of enantiomers: needs, challenges, perspectives☆

Efficient Separation of Enantiomers Using Stereoregular Chiral Polymers

Direct liquid chromatographic resolution of racemic drugs using α1-acid glycoprotein as the chiral stationary phase

Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.

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Affinity Chromatography: A Review of Clinical Applications

One of the most practically important functions of optically active polymers is chiral recognition. In the past two decades, various optically active polymers have been utilized as the chiral stati...

Optically Active Polymers for Chiral Separation

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : III. Optical resolution of a series of N-aroyl d,l-amino acids by high-performance liquid chromatography on bovine serum albumin covalently bound to silica

Direct resolution of enantiomers by liquid affinity chromatography on albumin-agarose under isocratic conditions

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : IV. Molecular interaction forces and retention behaviour in chromatography on bovine serum albumin as a stationary phase☆

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : II. Optical resolution of a sulphoxide, a sulphoximine and a benzoylamino acid

Björn Bomgren

Papers

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : III. Optical resolution of a series of N-aroyl d,l-amino acids by high-performance liquid chromatography on bovine serum albumin covalently bound to silica

Direct resolution of enantiomers by liquid affinity chromatography on albumin-agarose under isocratic conditions

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : IV. Molecular interaction forces and retention behaviour in chromatography on bovine serum albumin as a stationary phase☆

Direct liquid chromatographic separation of enantiomers on immobilized protein stationary phases : II. Optical resolution of a sulphoxide, a sulphoximine and a benzoylamino acid

Direct optical resolution of a series of pharmacologically active racemic sulfoxides by high-performance liquid affinity chromatography