Showing papers by "Brian Wigdahl published in 1982"
TL;DR: In this system a large fraction of the latently infected cells contain an HSV genome that can be activated, and cell survival was reduced minimally after repression of high multiplicity HSV type 1 (HSV-1) infection.
Abstract: Herpes simplex virus (HSV) exists in humans in a latent form that can be activated. To characterize the molecular basis of the cell-virus interactions and to analyze the state of the latent HSV genome, an in vitro model system was established. In this system a large fraction of the latently infected cells contain an HSV genome that can be activated. Cell survival was reduced minimally after repression of high multiplicity HSV type 1 (HSV-1) infection of human fibroblast cells with (E)-5-(2-bromovinyl)-2'-deoxyuridine in combination with human leukocyte interferon (IFN-alpha). A minimum of 1 to 3 percent of the surviving cells contained an HSV genome that could be activated either by human cytomegalovirus superinfection or reduction in incubation temperature.
59 citations
TL;DR: This system has provided the basis for further studies concerning the regulation of HSV gene expression in human cells and demonstrated that HSV-2 DNA synthesis is not required for activation.
20 citations