Showing papers in "Virology in 1982"

TL;DR: The viral glycoprotein GP-1 likely contains both the attachment and fusion activities of mouse hepatitis virus-4, as well as three viral polypeptide specificities characterized by indirect immunofluorescence and immune precipitation.

TL;DR: Results may indicate epitopes in close proximity or suggest allosteric modifications in the G-protein induced by antibody binding in vesicular stomatitis virus serotypes Indiana and New Jersey.

TL;DR: The reactivity of seal influenza virus and other H7 viruses from birds with the monoclonal antibodies show that some avian strains possess HA molecules that are closely related to those of seal virus and may therefore be related to viruses that were important in the evolution of this strain.

TL;DR: Immune clearing experiments were employed to demonstrate that this monoclonal antibody detects the same Elb-58Kd antigen observed previously with polyclonal antisera from hamsters bearing adenovirus-induced tumors.

TL;DR: Analysis of myxovirus particles devoid of the exposed portions of their spikes revealed that fatty acids are linked to the hydrophobic tail fragment of the glycoprotein which is associated with the viral lipid bilayer.

TL;DR: It was demonstrated that tsB5 enters human embryonic lung (HEL) cells more rapidly than KOS, another strain of HSV-1, and DNA sequences that specify a functional domain for virus entry were localized to the nucleotide sequences between the two mutations.

TL;DR: Using neutralization and hemagglutination inhibition (HI) testing, at least four distinct antigenic regions (epitopes) on the type 3 HA are defined and there appears to be marked functional specialization within regions of the reovirus type 3HA.

TL;DR: The results imply that MCFs and ecotropic MuLVs enter NIH/3T3 cells via different receptors and suggest that interference may have a profound effect on the dynamics of viral replication in vivo.

TL;DR: Eleven monoclonal antibodies reactive with the major surface glycoprotein of vesicular stomatitis virus serotypes Indiana and New Jersey were used to map antigenic sites found on one or both serotypes, but they were unable to neutralize infectivity of the virus in vitro although they were able to bind to the G-protein.

TL;DR: In this article, the authors characterized the viral-specific RNAs present in C127 mouse cells transformed by the BPV-1 virus or by cloned DNA and found that all five viral RNA species share a common 3′ terminus at 0.34 map units and the 5′ ends map at approximately 0.85 map units.

TL;DR: Comparative peptide mapping and direct C-terminal sequence analysis of intact NA and Pronase-released heads showed that the membrane-associated stalk region of the enzyme comes from the N-terminals of the molecule, suggesting that all sugar units are in N-glycosidic linkage to asparagine residues.

TL;DR: Cross protection apparently results from the prevention of uncoating of related challenge virus in light green tissue of N. sylvestris, which is much less susceptible than healthy tissue, to some extent, even to unrelated viruses.

TL;DR: An analysis has been made of the proteins synthesized in vivo and in vitro from two closely related tomato strains of tobacco mosaic virus, one of which is defective in cell- to-cell movement at a restrictive temperature, implicating this polypeptide as a factor mediating cell-to- cell movement of virus in the leaf.

TL;DR: The activation of cellular genes after infection of chick embryo fibroblasts (CEF) with temperature-sensitive mutants of herpes simplex virus type 1 with a mutation in the gene sequences which encode the immediate-early polypeptide Vmw175 is described.

TL;DR: It is found that the S4 double-stranded RNA segment (the segment that encodes the σ3 polypeptide, the major outer capsid polypeptic) is responsible for the capacity of type 2 to inhibit L cell RNA and protein synthesis.

TL;DR: This paper presents the first quantitative single cell analysis of the synthesis of viral nucleic acids, using improved methods of in situ hybridization, and identifies early visna virus DNA synthesis as the rate-limiting step in transcription, virus production, and cell death in vitro.

TL;DR: A two-site immunometric assay using monoclonal antibodies directed against two independent epitopes allows detection of isolated gp51 molecules at a minimal concentration of 0.4 ng/ml and is also suitable for the detection of BLV particles.

TL;DR: It appears that coordinate synthesis of vRNAs does not occur and comparisons between the genome of released virus and the composition of vRNA in the infected cells indicate that a mechanism exists for selecting the genome complement.

TL;DR: Sequence analyses of the 5′ and 3′ termini of the unpolyadenylated transcripts of influenza virus RNAs have shown that these molecules are exact complements with pppAp at their 5′ termina, indicating that their syntheses differ in both initiation and terminated transcription.

TL;DR: In the absence of the immune system, the MHV strains assayed exhibit differences in viral tropism, cytopathic changes, and viral assembly in CNS cells, and these differences may account for the different disease patterns.

TL;DR: Translation of an unfractionated mixture of genome RNA segments from calf rotavirus in a nuclease-treated rabbit reticulocyte system allowed the primary gene products of the viral genome to be defined.

TL;DR: Viral infection alters the cytoplasmic distribution of mitochondria consistent with the proposed role of vimentin filaments in determining the distribution of mitochondrial distribution in CV-1 cells.

TL;DR: Ten nuclear polyhedrosis viruses infecting insect hosts in the genus Heliothis and isolated in different geographical regions of the world were characterized using restriction endonuclease analysis, resulting in fragmentation profiles which separated the wild-type isolates into two major genotypes, corresponding with the morphology of the virion.

TL;DR: The biologic functions of hemagglutination and virus neutralization were primarily associated with only one antigenic epitope present on the gp56, and close spatial arrangement of gp56 and gp50 in the native virion could be demonstrated.

TL;DR: Plasmids containing hybrid EI regions from the nononcogenic adenovirus type 5 (Ad5) and the highly oncogenic Ad12 are capable of transforming baby rat kidney cells into cell lines with the typical adanovirus-transformed phenotype, indicating an extensive functional homology between the EI transcriptional units of the two serotypes at the level of transformation.

TL;DR: Analysis of field strains showed that antigenic variation in the neuraminidase was evident even between influenza virus isolated in the first year following the emergence of a new pandemic strain, suggesting that the distribution of neuramidase molecules may differ among viruses possessing N2 neuraminidsase.

TL;DR: The results are consistent with the idea that Friend leukemia virus actually causes disease via MCF intermediates, and indicate that DBA/2 cells are resistant to MCF infection in vitro and, by implication, toMCF-mediated leukemogenesis in vivo by a process analogous to viral interference.

TL;DR: Three of the eight antigenic sites on the bovine leukemia virus envelope gp51 were shown to be involved in the biological activities tested for in infectivity and syncytia neutralization tests, and can be the target for complement-dependent cytotoxicity.

TL;DR: The DNA of C6/SV40 mutant does not replicate detectably in permissive, simian cells, but it transforms established rat cells with an efficiency equal to that of wild-type DNA, although the phenotype of the mutant-transformed cells is somewhat different from that of the wild- type transformants.

TL;DR: Data suggest that leaves of subliminally infected plants support TMV replication in those cells which receive virus during mechanical inoculation, but that the infectious principle is unable to move from those original centers in these hosts.