B
Burt Zerner
Researcher at University of Queensland
Publications - 115
Citations - 6256
Burt Zerner is an academic researcher from University of Queensland. The author has contributed to research in topics: Carboxylesterase & Urease. The author has an hindex of 37, co-authored 115 publications receiving 6097 citations.
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Book ChapterDOI
Reassessment of Ellman's reagent.
Journal ArticleDOI
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
TL;DR: The molar absorption coefficient of the dianion of TNB is 14,150 at 412 nm in dilute aqueous salt solutions and this value was confirmed independently by reduction of purified DTNB with cysteine.
Journal ArticleDOI
Jack bean urease (ec 3.5.1.5), a metalloenzyme, a simple biological role for nickel
Journal ArticleDOI
Jack bean urease (EC 3.5.1.5). V. On the mechanism of action of urease on urea, formamide, acetamide, N-methylurea, and related compounds
TL;DR: A mechanism of action for urease is proposed which involves initially an O-bonded complex between urea and an active-site Ni2+ ion and subsequently anO-bonding carbamato-enzyme intermediate.
Journal ArticleDOI
Jack bean urease: the first nickel enzyme
Robert L. Blakeley,Burt Zerner +1 more
TL;DR: In this paper, the authors defined the development of their present understanding of the urease molecule and described physical and chemical properties of the enzyme, the interaction of inhibitors with the active site Ni(II) ion(s), substrate specificity and differential effects of pH on catalytic efficiency.