Showing papers in "Analytical Biochemistry in 1979"

TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.

TL;DR: This method (through the use of a citratelarsenite mixture added immediately after the molybdate reagent) is relatively sensitive, color stable, and has the advantage of being insensitive to any newly released phosphate.

TL;DR: Sodium salicylate has been found to serve as a satisfactory fluor for enhanced detection of radioactivity in polyacrylamide gels and gives linearity and sensitivity comparable to diphenyl oxazole.

TL;DR: The method of Lowry and coworkers combined the use of copper, as suggested by Herriott, with the Folin phenol method, which originated from the work of Wu, to produce a more reliable and sensitive protein analysis.

TL;DR: A highly sensitive stain for visualizing proteins in polyacrylamide gels that will be especially useful for analysis of patterns of proteins from tissue where attainment of the high specific activity of isotope labeling which is necessary to detect minor protein components is expensive, technically difficult or prohibited.

TL;DR: The molar absorption coefficient of the dianion of TNB is 14,150 at 412 nm in dilute aqueous salt solutions and this value was confirmed independently by reduction of purified DTNB with cysteine.

TL;DR: The method is quite specific and can detect as little as 5 pmol of ACC, the immediate precursor of ethylene in plant tissues, which is normally 80% and can be determined by internal standards.

TL;DR: The DNA concentration of a crude cellular homogenate can be measured accurately in the nanogram range using the fluorescence enhancement of 4′,6-diamidino-2-phenylindole (DAPI) or bisbenzimidazole (Hoechst H 33258) complexed with DNA.

TL;DR: Compared to other commonly used procedures this innovative and versatile technique can be conveniently applied to DNA microdetermination for the high sensibility/reproducibility ratio and can also be used without the need of previous purification steps.

TL;DR: Neuraminidase activity was considerably reduced in cultured fibroblasts of patients with mucolipidosis types I, II, and III and in cultured Fibroblast and leucocyte neuraminidases showed maximum activity between pH 4.2 and 4.4.

TL;DR: A new substrate for chymotrypsin, succinyl-Ala—Ala —Pro—Phe-p-nitroanilide has been synthesized and is readily soluble and stable to hydrolysis in Tris buffer at pH 8.0.

TL;DR: Furanacryloyl tripeptides conforming to the known substrate specificity of the angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1) have been employed to provide a continuous spectrophotometric assay for this peptidase in the visible region.

TL;DR: By this approach RNA molecules up to a size of 37 kb can be eluted undegraded with a recovery of 60–90% and eluted DNA can be correctly cleaved with restriction endonucleases as shown for λDNA using Eco RI.

TL;DR: The recommended method offers a consolidation of the recent modifications of the alditol acetate procedure for the estimation of neutral sugars and is applicable only to highly purified fiber preparations which are free of coprecipitated intracellular compounds.

TL;DR: In this article, the authors proposed a method for determining molecular weights from mobilities by direct calculation, based on plotting fragment length against reciprocal of mobility, which gives a straight line over a wider range than the conventional semilogarithmic plot.

TL;DR: An improved method of labeling collagen with [1- 14 C]acetic anhydride is described, which is used in a new assay for collagenase which is quick, sensitive, and reproducible.

TL;DR: The use of rat kidney instead of guinea pig brain as the source of histamine- N -methyltransferase for the enzymatic assay of histamines was found to improve the sensitivity of the assay.

TL;DR: A rapid sensitive method has been developed for the detection of protein bands in sodiumdodecyl sulfate-polyacrylamide gels that is suitable for the recovery of individual polypeptides on a preparative scale and allows for the complete recovery of unmodified protein from preparative gels.

TL;DR: This review article, with 58 references, evaluates studies in which the molecular size of an enzyme has been determined by means of ionizing radiation, finding that target theory analysis of these data has predicted the size of functional units and has been shown to apply to simple and complex enzymes.

TL;DR: Except for the peptidyl ethyl ester assay, all of the human leukocyte elastase assays reported in this paper are vastly more sensitive than any other existing assay for this protease.

TL;DR: An assay for simultaneous quantitation of deoxyribonucleoside triphosphates in cell extracts is described, which offers a simple and rapid alternative to either DNA polymerase or thin-layer chromatography assays for dNTPs in cellular extracts.

TL;DR: The procedure employs acetylation of the amino sugars at 25°C instead of at 0–1°C, resulting in five times the color intensity for galactosamine with no interference due to glucosamine.

TL;DR: Studies of the stability of stored blood samples indicate that whole blood, but not plasma, can be kept refrigerated for several days without significant loss of sulfhydryl reaction.

TL;DR: The recovered protein is suitable for N-terminal Edman degradation, tryptic peptide mapping, and amino acid analysis and can be renatured to regain enzymatic activity and antigenicity.

TL;DR: A reliable, simple, and rapid enzymatic method for the microdetermination of serum free fatty acids showed a close correlation with the chemical colorimetric method.

TL;DR: In this paper, a method utilizing metal chelate chromatography has been developed for the large-scale purification of two major plasma proteinase inhibitors under nondenaturing conditions, α 2 macroglobulin (α 2 M) and α 1 proteinase inhibitor (α 1 PI) are both isolated from the same batch of pooled human plasma by (NH 4 ) 2 SO 4 precipitation and chromatography on zinc chelate columns.

TL;DR: A method to quantitate collagen in solution based on binding of the dye Sirius Supra red F3BA by collagen followed by elution and estimation of the bound dye in a spectrophotometer is described.

TL;DR: A versatile hydroxylapatite batch assay for 1α,25-dihydroxyvitamin D3-receptor complex from chick intestinal mucosa makes it possible to examine a number of aspects of the binding of this steroid hormone to its cytoplasmic receptor that has not previously been possible.