I read this book the same weekend that the Packers took on the Rams, and the experience of the latter event, obviously, colored my judgment. Although I abhor anything that smacks of being a handbook (like, \"How to Earn a Merit Badge in Neurosurgery\") because too many volumes in biomedical science already evince a boyscout-like approach, I must confess that parts of this volume are fast, scholarly, and significant, with certain reservations. I like parts of this well-illustrated book because Dr. Sj6strand, without so stating, develops certain subjects on technique in relation to the acquisition of judgment and sophistication. And this is important! So, given that the author (like all of us) is somewhat deficient in some areas, and biased in others, the book is still valuable if the uninitiated reader swallows it in a general fashion, realizing full well that what will be required from the reader is a modulation to fit his vision, propreception, adaptation and response, and the kind of problem he is undertaking. A major deficiency of this book is revealed by comparison of its use of physics and of chemistry to provide understanding and background for the application of high resolution electron microscopy to problems in biology. Since the volume is keyed to high resolution electron microscopy, which is a sophisticated form of structural analysis, but really morphology in a modern guise, the physical and mechanical background of The instrument and its ancillary tools are simply and well presented. The potential use of chemical or cytochemical information as it relates to biological fine structure , however, is quite deficient. I wonder when even sophisticated morphol-ogists will consider fixation a reaction and not a technique; only then will the fundamentals become self-evident and predictable and this sine qua flon will become less mystical. Staining reactions (the most inadequate chapter) ought to be something more than a technique to selectively enhance contrast of morphological elements; it ought to give the structural addresses of some of the chemical residents of cell components. Is it pertinent that auto-radiography gets singled out for more complete coverage than other significant aspects of cytochemistry by a high resolution microscopist, when it has a built-in minimal error of 1,000 A in standard practice? I don't mean to blind-side (in strict football terminology) Dr. Sj6strand's efforts for what is \"routinely used in our laboratory\"; what is done is usually well done. It's just that …

Methods in Enzymology.

A study of a sample provides only an estimate of the true (population) value of an outcome statistic. A report of the study therefore usually includes an inference about the true value. Traditionally, a researcher makes an inference by declaring the value of the statistic statistically significant or nonsignificant on the basis of a P value derived from a null-hypothesis test. This approach is confusing and can be misleading, depending on the magnitude of the statistic, error of measurement, and sample size. The authors use a more intuitive and practical approach based directly on uncertainty in the true value of the statistic. First they express the uncertainty as confidence limits, which define the likely range of the true value. They then deal with the real-world relevance of this uncertainty by taking into account values of the statistic that are substantial in some positive and negative sense, such as beneficial or harmful. If the likely range overlaps substantially positive and negative values, they infer that the outcome is unclear; otherwise, they infer that the true value has the magnitude of the observed value: substantially positive, trivial, or substantially negative. They refine this crude inference by stating qualitatively the likelihood that the true value will have the observed magnitude (eg, very likely beneficial). Quantitative or qualitative probabilities that the true value has the other 2 magnitudes or more finely graded magnitudes (such as trivial, small, moderate, and large) can also be estimated to guide a decision about the utility of the outcome.

/pdf/making-meaningful-inferences-about-magnitudes-tgf5lnrwpu.pdf

Making meaningful inferences about magnitudes.

Exercise training is a clinically proven, cost-effective, primary intervention that delays and in many cases prevents the health burdens associated with many chronic diseases. However, the precise type and dose of exercise needed to accrue health benefits is a contentious issue with no clear consensus recommendations for the prevention of inactivity-related disorders and chronic diseases. A growing body of evidence demonstrates that high-intensity interval training (HIT)canserveasaneffectivealternatetotraditionalendurance-basedtraining,inducingsimilar or even superior physiological adaptations in healthy individuals and diseased populations, at least when compared on a matched-work basis. While less well studied, low-volume HIT can also stimulate physiological remodelling comparable to moderate-intensity continuous training despite a substantially lower time commitment and reduced total exercise volume. Such findings areimportantgiventhat'lackoftime'remainsthemostcommonlycitedbarriertoregularexercise participation. Here we review some of the mechanisms responsible for improved skeletal muscle metabolic control and changes in cardiovascular function in response to low-volume HIT. We also consider the limited evidence regarding the potential application of HIT to people with, or at risk for, cardiometabolic disorders including type 2 diabetes. Finally, we provide insight on the utility of low-volume HIT for improving performance in athletes and highlight suggestions for future research.

/pdf/physiological-adaptations-to-low-volume-high-intensity-3li4sknb0x.pdf

Physiological adaptations to low-volume, high-intensity interval training in health and disease

Tea polyphenols: prevention of cancer and optimizing health. Discussion 4.

An athlete’s carbohydrate intake can be judged by whether total daily intake and the timing of consumption in relation to exercise maintain adequate carbohydrate substrate for the muscle and central nervous system (‘‘high carbohydrate availability’’) or whether carbohydrate fuel sources are limiting for the daily exercise programme (‘‘low carbohydrate availability’’). Carbohydrate availability is increased by consuming carbohydrate in the hours or days prior to the session, intake during exercise, and refuelling during recovery between sessions. This is important for the competition setting or for high-intensity training where optimal performance is desired. Carbohydrate intake during exercise should be scaled according to the characteristics of the event. During sustained high-intensity sports lasting *1 h, small amounts of carbohydrate, including even mouth-rinsing, enhance performance via central nervous system effects. While 30–60 g h 71 is an appropriate target for sports of longer duration, events42.5 h may benefit from higher intakes of up to 90 g h 71 . Products containing special blends of different carbohydrates may maximize absorption of carbohydrate at such high rates. In real life, athletes undertake training sessions with varying carbohydrate availability. Whether implementing additional ‘‘train-low’’ strategies to increase the training adaptation leads to enhanced performance in well-trained individuals is unclear.

/pdf/carbohydrates-for-training-and-competition-ei43zrhkcx.pdf

Carbohydrates for training and competition.

We determined the effects of a cycle training program in which selected sessions were performed with low muscle glycogen content on training capacity and subsequent endurance performance, whole body substrate oxidation during submaximal exercise, and several mitochondrial enzymes and signaling proteins with putative roles in promoting training adaptation. Seven endurance-trained cyclists/triathletes trained daily (High) alternating between 100-min steady-state aerobic rides (AT) one day, followed by a high-intensity interval training session (HIT; 8 × 5 min at maximum self-selected effort) the next day. Another seven subjects trained twice every second day (Low), first undertaking AT, then 1–2 h later, the HIT. These training schedules were maintained for 3 wk. Forty-eight hours before and after the first and last training sessions, all subjects completed a 60-min steady-state ride (60SS) followed by a 60-min performance trial. Muscle biopsies were taken before and after 60SS, and rates of substrate oxidation were determined throughout this ride. Resting muscle glycogen concentration (412 ± 51 vs. 577 ± 34 μmol/g dry wt), rates of whole body fat oxidation during 60SS (1,261 ± 247 vs. 1,698 ± 174 μmol·kg−1·60 min−1), the maximal activities of citrate synthase (45 ± 2 vs. 54 ± 1 mmol·kg dry wt−1·min−1), and β-hydroxyacyl-CoA-dehydrogenase (18 ± 2 vs. 23 ± 2 mmol·kg dry wt−1·min−1) along with the total protein content of cytochrome c oxidase subunit IV were increased only in Low (all P < 0.05). Mitochondrial DNA content and peroxisome proliferator-activated receptor-γ coactivator-1α protein levels were unchanged in both groups after training. Cycling performance improved by ∼10% in both Low and High. We conclude that compared with training daily, training twice every second day compromised high-intensity training capacity. While selected markers of training adaptation were enhanced with twice a day training, the performance of a 1-h time trial undertaken after a 60-min steady-state ride was similar after once daily or twice every second day training programs.

Skeletal muscle adaptation and performance responses to once a day versus twice every second day endurance training regimens.

Performance tests are an integral component of assessment for competitive cyclists in practical and research settings. Cycle ergometry is the basis of most of these tests. Most cycle ergometers are stationary devices that measure power while a cyclist pedals against sliding friction (e.g. Monark), electromagnetic braking (e.g. Lode), or air resistance (e.g. Kingcycle). Mobile ergometers (e.g. SRM cranks) allow measurement of power through the drive train of the cyclist’s own bike in real or simulated competitions on the road, in a velodrome or in the laboratory. The manufacturers’ calibration of all ergometers is questionable; dynamic recalibration with a special rig is therefore desirable for comparison of cyclists tested on different ergometers.

Tests of cycling performance.

The race-to-race variation in performance of a top athlete determines the smallest change in performance affecting the athlete's chances of winning. We report here the typical variation in competition times of elite cyclists in various race series. Repeated-measures analysis of log-transformed official race times provided the typical variation in a cyclist's performance as a coefficient of variation. The typical variation of a top cyclist (and its 95% likely limits) was 0.4% (0.3–0.5%) in World Cup road races, 0.7% (0.7–0.8%) in Tour de France road races, 1.2% (0.8–2.2%) in the Kilo, 1.3% (0.9–2.4%) in road time trials, 1.7% (1.2–2.6%) in Tour de France time trials, and 2.4% (2.1–2.8%) in World Cup mountain biking. Cyclist interdependence arising from team tactics and pack riding probably accounts for the lower variability in performance of cyclists in road races and precludes estimation of the smallest worthwhile change in performance time for cyclists in these events. The substantial difference...

Variation in performance of elite cyclists from race to race

PATON, C. D., W. G. HOPKINS, and L. VOLLEBREGT. Little effect of caffeine ingestion on repeated sprints in team-sport athletes. Med. Sci. Sports Exerc., Vol. 33, No. 5, 2001, pp. 822–825. Purpose:The effect of caffeine ingestion on sprint performance is unclear. We have therefore investigated its ef

Little effect of caffeine ingestion on repeated sprints in team-sport athletes.

We have previously demonstrated that well-trained subjects who completed a 3 week training programme in which selected high-intensity interval training (HIT) sessions were commenced with low muscle glycogen content increased the maximal activities of several oxidative enzymes that promote endurance adaptations to a greater extent than subjects who began all training sessions with normal glycogen levels. The aim of the present study was to investigate acute skeletal muscle signalling responses to a single bout of HIT commenced with low or normal muscle glycogen stores in an attempt to elucidate potential mechanism(s) that might underlie our previous observations. Six endurance-trained cyclists/triathletes performed a 100 min ride at approximately 70% peak O(2) uptake (AT) on day 1 and HIT (8 x 5 min work bouts at maximal self-selected effort with 1 min rest) 24 h later (HIGH). Another six subjects, matched for fitness and training history, performed AT on day 1 then 1-2 h later, HIT (LOW). Muscle biopsies were taken before and after HIT. Muscle glycogen concentration was higher in HIGH versus LOW before the HIT (390 +/- 28 versus 256 +/- 67 micromol (g dry wt)(1)). After HIT, glycogen levels were reduced in both groups (P < 0.05) but HIGH was elevated compared with LOW (229 +/- 29 versus 124 +/- 41 micromol (g dry wt)(1); P < 0.05). Phosphorylation of 5 AMP-activated protein kinase (AMPK) increased after HIT, but the magnitude of increase was greater in LOW (P < 0.05). Despite the augmented AMPK response in LOW after HIT, selected downstream AMPK substrates were similar between groups. Phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) was unchanged for both groups before and after the HIT training sessions. We conclude that despite a greater activation AMPK phosphorylation when HIT was commenced with low compared with normal muscle glycogen availability, the localization and phosphorylation state of selected downstream targets of AMPK were similar in response to the two interventions.

https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/expphysiol.2009.049353

Carl D. Paton

Papers

Skeletal muscle adaptation and performance responses to once a day versus twice every second day endurance training regimens.

Tests of cycling performance.

Variation in performance of elite cyclists from race to race

Little effect of caffeine ingestion on repeated sprints in team-sport athletes.

Acute signalling responses to intense endurance training commenced with low or normal muscle glycogen.