Showing papers by "Carlos Henrique Gomes Martins published in 2023"
TL;DR: In this article , the effects of oleoresin obtained from the species Copaifera trapezifolia Hayne (CTO), and an isolated molecule found in the CTO, ent-polyalthic acid (ent-15,16-epoxy-8(17),13(16),14-labdatrien-19-oic acid) (named as PA), against T. gondii infection were assessed.
TL;DR: In this paper , the synthesis and crystal structures of two carbazate ligands, 2-hydroxyacetophenone-methylcarbazate (HL1), and their Cu(II) complexes [Cu(L1)(CH3OH)]2 (1) and [cu(L2)]n (2), were characterized by X-ray single-crystal analysis, spectroscopic and physicochemical methods.
TL;DR: In this article , the authors investigated the effects of the leaf hydroalcoholic extract and oleoresin from Copaifera multijuga against Toxoplasma gondii in human villous (BeWo) and extravillous (HTR8/SVneo) trophoblast cells, as well as in adult villous explants from third-trimester pregnancy.
Abstract: The conventional treatment of congenital toxoplasmosis is mainly based on the combination of sulfadiazine and pyrimethamine. However, therapy with these drugs is associated with severe side effects and resistance, requiring the study of new therapeutic strategies. There are currently many studies with natural products, including Copaifera oleoresin, showing actions against some pathogens, as Trypanosoma cruzi and Leishmania. In the present study, we investigated the effects of the leaf hydroalcoholic extract and oleoresin from Copaifera multijuga against Toxoplasma gondii in human villous (BeWo) and extravillous (HTR8/SVneo) trophoblast cells, as well as in human villous explants from third-trimester pregnancy. For this purpose, both cells and villous explants were infected or not with T. gondii, treated with hydroalcoholic extract or oleoresin from C. multijuga and analyzed for toxicity, parasite proliferation, cytokine and ROS production. In parallel, both cells were infected by tachyzoites pretreated with hydroalcoholic extract or oleoresin, and adhesion, invasion and replication of the parasite were observed. Our results showed that the extract and oleoresin did not trigger toxicity in small concentrations and were able to reduce the T. gondii intracellular proliferation in cells previously infected. Also, the hydroalcoholic extract and oleoresin demonstrated an irreversible antiparasitic action in BeWo and HTR8/SVneo cells. Next, adhesion, invasion and replication of T. gondii were dampened when BeWo or HTR8/SVneo cells were infected with pretreated tachyzoites. Finally, infected and treated BeWo cells upregulated IL-6 and downmodulated IL-8, while HTR8/SVneo cells did not change significantly these cytokines when infected and treated. Finally, both the extract and oleoresin reduced the T. gondii proliferation in human explants, and no significant changes were observed in relation to cytokine production. Thus, compounds from C. multijuga presented different antiparasitic activities that were dependent on the experimental model, being the direct action on tachyzoites a common mechanism operating in both cells and villi. Considering all these parameters, the hydroalcoholic extract and oleoresin from C. multijuga can be a target for the establishment of new therapeutic strategy for congenital toxoplasmosis.
TL;DR: In this paper , the authors evaluated the antioxidant, antibacterial activity, and the total phenols and tannins present in the ethanolic extract of Stryphnodendron polyphyllum.
Abstract:
El polen de Stryphnodendron polyphyllum (Mart.), típicamente conocido como barbatimão, es un problema para los apicultores con apiarios cerca de la planta, debido a toxicidad del polen para las abejas. El objetivo fue evaluar la actividad antioxidante, antibacteriana, determinar fenoles totales y taninos en el extracto etanólico. La actividad antioxidante se evaluó por método DPPH. La actividad antibacteriana se realizó por método de microdilución. Los taninos totales y fenoles se determinaron por método espectrofotométrico. Tuvimos resultados con actividad inhibitoria y bactericida para Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius y Lactobacillus paracasei. Se determinó EC50 de 7.42mg/mL en la actividad antioxidante. Para compuestos fenólicos y tánicos los resultados comparados con la curva de calibración son 3,12±0,005g/100g de muestra expresados como ácido gálico y 7,3±0,03g/100g de muestra expresados como ácido tánico.
Pollen from the Stryphnodendron polyphyllum (Mart.), typically known as barbatimão, is a problem for beekeepers who have apiaries close to the plant, due to pollen toxicity for bees. The objective was to evaluate the antioxidant, antibacterial activity, determine the total phenols and tannins present in the ethanolic extract. Antioxidant activity was evaluated by the DPPH method. Antibacterial activity was performed by the microdilution method.Total tannins and phenols were determined by the spectrophotometric method. We had results with inhibitory and bactericidal activity for Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius and Lactobacillus paracasei. It was determined EC50 of 7.42mg/mL in the antioxidant activity. For phenolic and tannic compounds the results compared to the calibration curve are 3.12±0.005g/100g of sample expressed as gallic acid and 7.3±0.03g/100g of sample expressed as tannic acid.