Carol A. Mayeda

TL;DR: A transposon-based DNA sequencing strategy that allows the introduction of sequencing priming sites throughout a target sequence by bacterial mating is described, and a miniplasmid was designed to select against transposition insertions into the vector.

TL;DR: The strategy used in sequencing SEQ89E and an analysis of its open reading frames are presented and principal findings include a putative ABD-A protein larger than a previously known ABD -A protein and aPutative glucose transporter-like gene located at or near the bithoraxoid (bxd), infra-abdominal-2 (iab-2) boundary on the opposite strand relative to that of the homeobox-containing genes.

TL;DR: A modified P element transposon is mobilized that carries a bacterial plasmid origin of replication and a drug-resistance marker and is mobilize as a plasmids in Escherichia coli to screen for transposons insertions in or near Drosophila loci that correspond to cloned DNA sequences.

TL;DR: A comparative approach to investigate both the regulation and the evolution of the largest Sgs-3-homologous glue genes in the Drosophila melanogaster genome found several conserved sequence elements in regions known to be involved in regulation of the gene represent potential sites of cis-acting regulatory sequences.

TL;DR: Two regulatory regions required for expression of reporter genes from the Sgs-3 promoter are defined and can confer a salivary gland-specific pattern of expression on a heterologous promoter and are also sufficient to drive gene expression in other Drosophila species, implying conservation of regulators.